目的 研究葎草Humulus scandens茎和叶中的化学成分。方法 应用正、反相硅胶柱色谱，半制备HPLC等色谱手段进行分离纯化；根据理化性质和波谱学方法鉴定化合物的结构。结果 从葎草茎和叶甲醇提取物的醋酸乙酯萃取物中分离得到了10个化合物，分别鉴定为N-p-香豆酰酪胺（1）、N-反式-阿魏酰基酪胺（2）、大波斯菊苷（3）、3，3’-dimethoxy-4，8’-oxyneoligna-9，4’，7’，9’-tetraol（4）、3-hydroxy-5，6-epoxy-7-megastigmen-9-one（5）、4，5-dihydroblumenol（6）、东莨菪素（7）、柑桔素C（8）、苯乙醇-O-β-D-吡喃葡萄糖苷（9）、3-oxo-α-ionol-β-D-glucopyranoside（10）。生物活性研究发现化合物2、4、7和9对DPPH的IC₅₀分别为0.01、3.36、10.55和18.15 μg/mL。结论 化合物2、4～6、8～10为首次从该植物中分离得到，化合物2、4～6、9和10为首次从葎草属植物中分离得到，化合物2、4、7和9具有较好的自由基清除活性，可为开发抗氧化和抗老化方面的药物先导化合物提供科学依据。

Objective To study the chemical constituents from the roots and stems of Humulus scandens. Methods The chromatographies on silica gel column, reverse phase column, and semi-preparative HPLC were used to isolate and purify the constituents and the structures of the compounds were elucidated on the basis of physicochemical properties and spectral data. Results Ten compounds were obtained from the ethyl acetate fraction of methanol extract in the roots and stems of H. scandens and identitied as N-p-coumaroyltyramine (1), N-trans-feruloyltyramine (2), cosmosiin (3), 3, 3'-dimethoxy-4, 8'-oxyneoligna-9, 4', 7', 9'-tetraol (4), 3-hydroxy-5, 6-epoxy-7-megastigmen-9-one (5), 4, 5-dihydroblumenol (6), scopoletin (7), citruusin C (8), 2-phenylethyl-O-β-D-glucopyranoside (9), and 3-oxo-α-ionol-β-D-glucopyranoside (10). Conclusion Compounds 2, 4—6, and 8—10 are all obtained from H. scandens for the first time, and compounds 2, 4—6, 9, and 10 are firstly isolated from the plants of this genus. Compounds 2, 4, 7, and 9 show remarkable scavenging activity toward DPPH radical with IC₅₀ values of 0.01, 3.36, 10.55, and 18.15 μg/mL, respectively. The scientific evidence is provided for the development of anti-oxidant and anti-aging aspects of the drugs.

国家自然科学基金资助项目（20962020/B0204）