目的 建立测定金银花药材中新绿原酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸A、异绿原酸B、异绿原酸C、断氧化马钱子苷8种成分的HPLC方法。方法 采用RP-HPLC法，色谱柱为Luna 5 μm C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为甲醇（A）-0.1%磷酸水（B）溶液，梯度洗脱：0～20 min，12%～30% A；20～60 min，30%～50% A，体积流量1.0 mL/min；检测波长237、324 nm，柱温30 ℃。结果 8种成分均达到基线分离，各成分均有较宽的线性范围和良好的线性关系（r＞0.999 9）；回收率在98.72%～102.50%。结论 建立的测定方法准确灵敏、重复性好，能较全面地评价金银花药材的质量。

Objective To establish an HPLC method for simultaneously determining eight components such as neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, and secoxyloganin in Lonicerae Japonicae Flos. Methods A RP-HPLC method was established with Luna C₁₈ column (250 mm × 4.6 mm, 5 μm) by a gradient elution using methanol (A) and 0.1% phosphoric acid (B): 0－20 min, 12%－30% A; 20－60 min, 30%－50% A; The flow rate was 1.0 mL/min with double wavelength (237 and 324 nm), and the column temperature was 30 ℃. Results The eight components was separated to baseline; Each component had a wide linear range and a good linear relationship (r > 0.999 8); The recoveries were 98.72% to 102.50%. Conclusion The method is accurate, sensitive, reliable, and has a good reproducibility. It could be used for the quality control of Lonicerae Japonicae Flos.

国家科技部国家重大新药创制专项“现代中药创新集群与数字制药技术平台”（2013ZX09402203）