目的 制备荆芥油-苦参素（OMT）阴道用脂质体温敏凝胶并考察其体外释放特征。方法 以泊洛沙姆407（P-407）和泊洛沙姆188（P-188）为凝胶基质，以凝胶的胶凝温度为考察指标对处方进行优化；复乳法制备OMT脂质体，冷溶法制备荆芥油-OMT阴道用脂质体温敏凝胶；采用HPLC法测定OMT的量，透析法研究荆芥油-OMT脂质体温敏凝胶的体外释放特性。结果 温敏凝胶基质最佳处方为18% P-407、5% P-188和0.2% 羟丙基甲基纤维素（HPMC），所制备的荆芥油-OMT阴道用脂质体温敏凝胶胶凝温度为（36.8±0.2）℃。体外释放结果显示，荆芥油-OMT阴道用脂质体温敏凝胶24 h内累积释放率为（58.89±0.34）%，48 h内累积释放率为（66.38±0.12）%，具有明显的缓释作用。结论 制得的荆芥油-OMT脂质体温敏凝胶具有温敏特性和缓释作用，能有效延缓药物在阴道中的释放速率，提高其在阴道中的滞留时间。

Objective To prepare the nepeta oil-oxymatrine (OMT) lipidosome pro vagina thermosensitive gel, and to investigate its in vitro drug release behavior. Methods P-407 and P-188 were used as gel matrix to prepare the gel, and gelatinization temperature was applied as a target to optimize the prescription. The OMT lipidosome was prepared based on the multiple emulsion method, and the nepeta oil-OMT lipidosome pro vagina thermosensitive gel was obtained by cold-dissolving method. The content of OMT was determined by HPLC, and in vitro release properties of nepeta oil-OMT lipidosome thermosensitive in situ gel was investigated by dialysis method. Results After optimization, the gel prescription was finally confirmed as 18% P-407, 5% P-188, and 0.2% hydroxy-propyl methyl cellulose (HPMC). The gelatination temperature for nepeta oil-OMT lipidosome thermosensitive gel was (36.8 ± 0.2) ℃, and the in vitro accumulating release ratio of sinomenine in the nepeta oil-OMT lipidosome gel system was (58.89 ± 0.34) % and (66.38 ± 0.12) % after 48 h. Conclusion The prepared nepeta oil-OMT lipidosome thermosensitive gel has the temperature sensitivity and sustained release effect, can effectively delay the release of the drug in vagina and improve the residence time in the vagina.

陕西省教育厅产业培育项目（2010JC20）；陕西省重点科技创新团队：基于秦巴优势中草药资源的新药创制创新团队（2013KCT-24）