目的 建立一种结合高效液相色谱飞行时间质谱（HPLC/TOF-MS）及高效液相色谱离子阱多级质谱（HPLC/IT-MSⁿ）对补骨脂药材进行化学成分定性分析的方法。方法 HPLC色谱分离采用Phenomenex luna C₁₈（5 μm）反相色谱柱为分析柱，0.1%甲酸水溶液-乙腈为流动相，梯度洗脱，检测波长为254 nm；质谱定性采用飞行时间质谱结合离子阱多级质谱，正负离子模式扫描。结果 在优化的液质联用条件下，飞行时间质谱筛查出13种成分的分子式，采用离子阱多级质谱鉴定分析此13种成分。结论 HPLC/TOF-MS和HPLC/IT-MSⁿ联合的分析方法，为中药的多成分分析提供了一种有效、可靠的新模式，此模式也同样适用于其他复杂体系的分析。

Objective To establsh a combinative HPLC/TOF-MS and HPLC/IT-MSⁿ method for quantitative analysis on chemical constituents in Psoraleae Fructus. Methods Separation was performed on a Phenomenex Luna C₁₈ (5 μm) reverse-phase column. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute. The flow rate was 1 mL/min. A combination of time-of-flight mass spectrometer (TOF-MS) and ion trap tandem mass spectrometer (IT-MSⁿ) was applied for qualitative analysis under positive and negative ion mode. Results Under optimized LC/MS condition, the molecular formulae of 13 components were screened by TOF-MS and all of them were then identified by IT-MSⁿ. Conclusion The established combinative HPLC/MS method could thus be considered as an effective and credible pattern for the analysis on the multi-components of Chinese materia medica and it also could be used for as the analysis on other complex systems.