目的 研究麻花头属植物麻花头Serratula chinensis干燥块根的化学成分。方法 采用硅胶、Sephadex LH-20、ODS柱色谱和制备HPLC等方法进行分离，并通过理化常数和NMR等各种波谱学方法鉴定化合物结构。结果 从广升麻干燥块根95%乙醇提取物的正丁醇部位分离得到了16个化合物，分别鉴定为20-羟基蜕皮甾酮（1）、polypodine B（2）、carthamosterone（3）、20, 22-异丙叉基-20-羟基蜕皮甾酮（4）、24-epi-abutasterone（5）、polypodine C（6）、coronatasterone（7）、20-羟基蜕皮甾酮2-O-β-D-葡萄糖苷（8）、20-羟基蜕皮甾酮25-O-β-D-葡萄糖苷（9）、polypodine B 20, 22-acetonide（10）、shidasterone（11）、2-O-乙酰基-20-羟基蜕皮甾酮（12）、3-O-乙酰基-20-羟基蜕皮甾酮（13）、20-羟基蜕皮甾酮-20, 22-缩丁醛（14）、24-methylene-shidasterone（15）及ajugasterone D（16）。结论 化合物2、4、5、7～10、12、15、16为首次从该植物中分离得到，化合物5、7～10、16为首次从麻花头属植物中分离得到。

Objective To study the chemical constituents from the roots tubers of Serratula chinensis. Methods The chemical constituents were separated and purified by silica gel, Sephadex LH-20, ODS column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties and spectral data. Results Sixteen compounds (ecdysteroids) were isolated from the n-butanol fraction of 95% ethanol extract in the root tubers of S. chinensis, and their structures were identified as 20-hydroxyecdysone (1), polypodine B (2), carthamosterone (3), 20-hydroxyecdysone-20, 22-monoacetonide (4), 24-epi-abutasterone (5), polypodine C (6), coronatasterone (7), 20-hydroxyecdysone 2-O-β-D-glucopyranoside (8), 20-hydroxyecdysone 25-O-β-D- glucopyranoside (9), polypodine B 20, 22-acetonide (10), shidasterone (11), 2-O-acetyl-20-hydroxyecdysone (12), 3-O-acetyl-20- hydroxyecdysone (13), 20-hyderoxyecdysone-20, 22-butylidene acetal (14), 24-methylene-shidasterone (15), and ajugasterone D (16). Conclusion Compounds 2, 4, 5, 7—10, 12, 15, and 16 are isolated from this plant for the first time, and compounds 5, 7—10, and 16 are found in the plants of genus Serratula L. for the first time.

国家药品标准提高暨2015版药典科研任务项目（YDC-113）