目的 对西藏不同产地17份麻花艽Gentiana straminea样品的遗传多样性进行比较分析。方法 居群采样，以相同产地同属近缘种粗茎秦艽G. crassicaulis为外类群比对，nrDNA ITS区序列分析，构建UPGMA系统树。结果 西藏产麻花艽nrDNA ITS区序列有1个多态性位点；外类群粗茎秦艽6份样品序列完全一致。结论 麻花艽与外类群种间区别明显；17份不同产地麻花艽被划分为2类，遗传多样性较为丰富，为西藏麻花艽药材品质评价及麻花艽核心种质的构建提供基础资料。

Objective To study the genetic diversity of 17 samples of Gentiana straminea from different habitats of Tibet. Methods Sampling in populations was carried out, and the related species G. crassicaulis from the same area was as the out group; nr DNA ITS sequences were amplified and analyzed; UPGMA systematic tree was constructed. Results One polymorphic site of G. straminea from Tibet was found; the six samples of out group have the identical DNA ITS sequences. Conclusion The difference between G. straminea and the out group is obvious; seventeen samples of G. straminea from different habitats are divided into two groups; genetic diversity is more abundant; the result could provide the basic reference for the quality evaluation and the construction of core germplasm of G. straminea in Tibet.

国家自然科学基金资助项目（81173654）