目的 探讨姜黄素对人肺腺癌A549细胞亚群SP及NON-SP生长的影响及其作用靶点。方法 从A549细胞中分选出SP及NON-SP细胞，通过软琼脂克隆形成实验及体外致瘤性对其进行鉴定。将SP及NON-SP细胞用不同浓度的姜黄素（10、20、30、40 μmol/L）处理，MTT法检测姜黄素对SP及NON-SP细胞生长的抑制作用；荧光定量PCR法检测细胞中survivin、caspase-3基因表达。结果 不同浓度的姜黄素对SP、NON-SP细胞的生长产生不同程度的抑制作用，浓度为30 μmol/L的时细胞生存率最低，且与作用时间呈正相关，对SP细胞的抑制作用明显强于NON-SP细胞。姜黄素抑制survivin基因的表达，促进caspase-3基因的表达，其对SP细胞凋亡的诱导作用明显强于NON-SP细胞。结论 SP细胞的致瘤性高于NON-SP细胞。姜黄素主要通过抑制SP细胞的生长、下调survivin基因的表达和促进caspase-3基因的表达而产生诱导肿瘤细胞凋亡的作用。

Objective To evaluate the effect of curcumin on A549 tumor cell growth of subsets SP and NON-SP cells and the specific targets. Methods The subsets SP and NON-SP cells were separated from A549 tumor strain of the human lung adenocarcinoma, and these cells were identified with soft agar clone formation test and tumorigenicity in vitro. Curcumin with different concentration (10, 20, 30, and 40 μmol/L) was used to deal with these cells. Using MTT method to make sure the inhibition of curcumin on the growth of SP and NON-SP cells. PCR was used to measure the expression of survivin and caspase-3. Results Curcumin at different concentration showed the different inhibition on SP and NON-SP cells. Curcumin (30 μmol/L) showed more inhibition on SP cells than NON-SP cells with the lowest cell viability and was time-dependent. Curcumin could suppress the expression of survivin and promote the expression of caspase-3. The apoptosis degree of SP cell was clearly higher than that of NON-SP cell. Conclusion Tumorigenicity of SP cell is higher than that of NON-SP cell. Curcumin induces the apoptosis of tumor cells through inhibiting the growth of SP cell, down-regulating the expression of survivin, and promoting the expression of caspase-3 gene.

国家自然科学基金资助项目（81001578）