目的 采用RP-HPLC法同时测定阿胶中13种氨基酸的量。方法 阿胶以6 mol/L盐酸于120 ℃水解3 h，以异硫氰酸苯酯柱前衍生，用反相高效液相色谱法测定氨基酸的量。采用Agilent Eclipse AAA色谱柱（75 mm×4.6 mm，3.5 μm），以乙腈-110 mmol/L醋酸胺溶液（1∶99）为流动相A、醋酸-乙腈-水（0.05∶80∶20）为流动相B进行梯度洗脱，体积流量0.6 mL/min，柱温42 ℃，检测波长为254 nm。结果 在36 min内可完成对阿胶中13种氨基酸的分离测定，各氨基酸的峰面积与浓度的线性关系良好（r＞0.997 7），加样回收率为95.2%～104.4%，RSD＜5.0%。结论 方法简便、准确，重复性好，可用于阿胶的质量控制，亦可为其他药物中氨基酸的分析提供参考。

Objective To establish a RP-HPLC method for the simultaneous determination of 13 amino acids in Asini Corii Colla. Methods Asini Corii Colla was hydrolyzed for 3 h at 120 ℃ using 6 mol/L hydrochloric acid, and the amino acids were determined by RP-HPLC after being derived with phenyl isothiocyanate. HPLC was performed on an Eclipse AAA column (75 mm × 4.6 mm, 3.5 ?m) with acetonitrile-110 mmol/L ammonium acetate solution (1 : 99) as mobile phase A and acetic acid-acetonitrile-water (0.05 : 80 : 20) as mobile phase B in the gradient mode at a flow rate of 0.6 mL/min. The column temperature was 42 ℃, and the detection wavelength was set at 254 nm. Results The 13 amino acids were separated within 36 min with a good linearity (r > 0.9977) in the range of the test concentration. The average recoveries (n = 3) were 95.2%—104.4% and the RSD values were less than 5.0%. Conclusion The method is simple, accurate, and repeatable, which could be available for the quality control of Asini Corii Colla. It may also serve as a good reference for the determination of amino acids in other pharmaceuticals.