目的 建立HPLC-ELSD法测定丹参及其提取物中Na、K元素的方法。方法 色谱柱为Waters Sepherisorb SCX柱（250 mm×4.6 mm，5 μm）；流动相为0.05 mol/L醋酸铵（醋酸调节pH值至4.8）-乙腈（70∶30），体积流量为1.0 mL/min，柱温30 ℃；ELSD漂移管温度100 ℃，载气体积流量2 L/min，检测器增益为2。结果 Na元素在19.7～197.0 μg/mL（r＝0.999 8）线性关系良好，K元素在35.0～350.0 μg/mL（r＝0.999 5）线性关系良好；Na、K平均回收率分别为99.0%、100.2%，RSD分别为0.7%、0.8%。结论 所建立的方法简单可靠，可以用于测定丹参及其提取物中Na、K元素。

Objective To establish the HPLC-ELSD method for the determination of sodium (Na) and potassium (K) in Salviae Miltiorrhizae Radix et Rhizoma (SMRR) and its extract. Methods The column was Waters Sepherisorb SCX column (250 mm × 4.6 mm, 5 μm); The mobile phase was 0.05 mol/L ammomium acetate solution (adjusted to pH 4.8 with acetic acid)-acetonitrile (70∶30); The flow rate was 1.0 mL/min; The column temperature was 30 ℃; The drift tube temperature of ELSD was 100 ℃; The gas flow rate was 2 L/min; And the detector gaining was 2. Results The calibration curves of Na and K were linear in the ranges of 19.7－197 μg/mL (r = 0.999 8) and 35.0－350 μg/mL (r = 0.999 5), respectively. The average recoveries of Na and K were 99% (RSD = 0.7%) and 100.2% (RSD = 0.8%), respectively. Conclusion The method is simple and accurate, and is suitable for determining the contents of Na and K in SMRR and its extract.

科技重大专项“重大新药创制”（2012ZX09101202）