目的 优化珐菲亚组织培养快速繁殖技术。方法 分别以MS和1/2MS为基本培养基，采用正交设计研究植物生长调节剂多因素组合（6-BA、NAA、IBA和IAA）对珐菲亚继代增殖和诱导生根的影响。结果 MS＋6-BA 1.5 mg/L＋NAA 0.1 mg/L＋IBA 0.2 mg/L利于丛生芽继代增殖，30 d繁殖系数6.0以上；1/2 MS＋NAA 1.0 mg/L＋IBA 0.2 mg/L适于诱导生根获得再生植株，生根率100%；生根苗移栽于排水良好的沙床上，成活率98%。结论 本实验为保护珐菲亚的野生资源，发展人工栽培和探讨育种新途径奠定基础。

Objective To establish and optimize the technique of tissue culture and rapid propagation of Pfaffia paniculata. Methods Using MS and 1/2MS basic media, the effects of various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seedlings subculture and rooting culture were studied by orthogonal design. Results The effective medium for cluster buds-inducing and subculture was MS + 6-BA 1.5 mg/L + NAA 0.1 mg/L + IBA 0.2 mg/L, and the propagation coefficient was over 6.0 per 30 d; The best rooting medium was 1/2 MS + NAA 1.0 mg/L + IBA 0.2 mg/L, and the rooting rate was 100%. The rooting seedlings were transplanted in well-drained sand bed and the survival rate was 98%. Conclusion The method in the experiment could provide a basis on protecting the mild resources of P. paniculata exploring the artificial resources, and discussing the new way breedings.

广西壮族自治区卫生厅科研资助项目（Z2006178）