目的 通过观察附子、仙茅、肉桂、黄柏、栀子对阳虚模型大鼠下丘脑-垂体-靶腺轴各项功能指标的影响，揭示在阳虚状态下辛热药、苦寒药不同药性的生物学表征特点。方法 辛热药附子、仙茅、肉桂和苦寒药黄柏、栀子水煎液每天ig给予阳虚模型大鼠1次，连续给药7 d，检测各组大鼠血清中促甲状腺激素（TSH）、三碘甲腺原氨酸（T₃）、甲状腺素4（T₄）、17-羟皮质类固醇（17-OHCS）、皮质醇（COR）、睾酮（T）、雌二醇（E₂）等指标的变化。结果 附子、仙茅、肉桂组均能显著升高阳虚大鼠T₃、17-OHCS、COR、T、T/E₂值，降低E₂指标；附子、肉桂组显著升高阳虚大鼠T₄水平，肉桂组显著降低阳虚大鼠TSH水平，黄柏、栀子组显著降低阳虚大鼠COR水平，黄柏组显著降低阳虚大鼠E₂指标。结论 辛热药附子、仙茅、肉桂和苦寒药黄柏、栀子在干预阳虚大鼠时表现出不同的药性，其中辛热药附子、仙茅、肉桂对阳虚状态的下丘脑-垂体-靶腺轴指标具有明显调节作用，而苦寒药黄柏、栀子无明显作用。

Objective To initially explore the drug biological characteristics of pungent-hot and bitter-cold herbs on the various functions of hypothalamus-hypophysis-target gland axises in Yang-deficiency rats, according to the pungent-hot herbs, Aconiti Lateralis Radix Praeparata (ALRP), Curculiginis Rhizoma (CR), Cinnamomi Cortex (CC), and bitter-cold Phellodendri Chinensis Cortex (PCC), and Gardeniar Fructus (GF) and to investigate their biological expression characteristic on Yang-deficiency state. Methods The decoctions of ALPR, CR, CC, PCC, and GF were ig administered to Yang-deficiency rats once daily for 7 d. The indexes of thyroid-stimulating hormone (TSH), triiodothyronine (T₃), tetraiodothyronine (T₄), 17-hydroxycorticosteroids (17-OHCS), cortisol (COR), testosterone (T), and estradiol (E₂) in serum of animals in each group were detected. Results The T₃, 17-OHCS, COR, T, and T/E₂ values of Yang-deficiency rats in ALRP, CR, and CC groups were obviously increased, while E₂ was decreased. ALRP and CC could significantly increase T₄ of Yang-deficiency rats, TSH in Yang-deficient rats was decreased by CC, the COR of Yang-deficiency rats in CC and GF groups was decreased, and E₂ in Yang-deficiency rats was decreased by CC. Conclusion The pungent-hot herbs of ALRP, CR, and CC and bitter-cold herbs of PCC, GF show the different drug property in intervention of Yang-deficiency rats. The pungent-hot herbs have an obvious regulation on hypothalamus-hypophysis-target gland axises, while the bitter-cold herbs have no significant role.

国家重点基础研究发展计划（“973”计划）项目（2007CB512605）；高等学校博士学科点专项科研基金博导类资助课题（20100013110011）；高等学校博士学科点专项科研基金新教师类资助课题（20120211120046）；甘肃省科技计划资助（1208RJYA056）；兰州大学自然科学类“中央高校基本科研业务费专项资金”自由探索项目-面上项目（lzujbky-2012-151，lzujbky-2012-153）