目的 研究肺筋草Aletris spicata全草的化学成分。方法 利用正、反相硅胶柱色谱、薄层色谱、Sephadex LH-20柱色谱及半制备高效液相色谱等方法进行分离纯化，然后根据理化性质和波谱数据鉴定化合物的结构，并对其中的三萜类化合物（化合物1～3）进行了抗肿瘤活和抗菌活性初步筛选。结果 从肺筋草的干燥全草中分离得到了9个化合物，分别鉴定为24-甲基-9, 19-环羊毛甾-24-烯-3-醇（1）、24-甲基-9, 19-环羊毛甾-25-烯-3-醇（2）、24, 24-二甲基-环木菠萝烷-3-醇（3）、美商陆酚A（4）、异美商陆酚A（5）、9′-methyl americanol A（6）、1-(4′-羟基苯基)-7-(3″-甲氧基-4″-羟基苯基)-4-烯-3-庚酮（7）、methyl-9, 12, 13-trihydroxyoctadeca-10E, 15Z-dienoate（8）、5-羟甲基-2-呋喃甲醛（9）。结论 所有化合物均为首次从该属植物中分离得到。化合物1～3无抗肿瘤和抗菌活性。

Objective To study the chemical constituents of Aletris spicata. Methods The compounds were isolated and repeatedly purified by silica gel, TLC, and Sephadex LH-20 column chromatography as well as semi-Prep HPLC, and their structures were elucidated by means of spectral analysis and physicochemical properties. The antitumor and antibacterial activities of triterpenoids (compounds 1—3) were primarily screened. Results Nine compounds were obtained and identified as 24-methyl-9, 19-cyclolanost- 24-en-3-ol (1), 24-methyl-9, 19-cyclolanost-25-en-3-ol (2), cycloneolitsol (3), americanol A (4), isoamericanol A (5), 9′-methyl americanol A (6), 1-(4′-hydroxyphenyl)-7-(3″-methoxyl-4″-hydroxyphenyl)-heptene-4-en-3-one (7), methyl-9, 12, 13-trihydroxy- octadeca-10E, 15Z-dienoate (8), and 5-hydroxymethyl-2-furancarboxaldehyde (9). Conclusion All the compounds are isolated from the plants in Aletris L. for the first time. No anti-tumor and anti-bacterial activities are found in compounds 1—3.

国家自然基金项目（81160390）；贵州省优秀青年科技人才培养计划项目（黔科合人字 [2011] 33号）；贵州省优秀科技教育人才省长专项资金项目（黔省专合字 [2010] 71号）