目的 研究罗汉果甜苷含药血清对大鼠肝星状细胞HSC-T6的毒性以及对细胞增殖与相关基因表达的影响，初步探讨其抗肝纤维化作用机制。方法 以罗汉果甜苷含药血清干预HSC-T6，乳酸脱氢酶（LDH）法检测罗汉果甜苷含药血清对HSC-T6的细胞毒性；MTT法检测其对HSC-T6生长的抑制作用；RT-PCR法检测HSC-T6中I型胶原（Col-I）、转化生长因子-β1（TGF-β1）及基质金属蛋白酶抑制因子-1（TIMP-1）mRNA表达。结果 罗汉果甜苷含药血清对HSC-T6无特异毒性，罗汉果甜苷含药血清体积分数为20%、10%时可显著抑制HSC-T6的增殖（P＜0.05、0.01），并对Col-I、TGF-β1及TIMP-1 mRNA表达均表现显著抑制作用（P＜0.05、0.01）。结论 罗汉果甜苷含药血清对HSC-T6细胞无特异毒性，对HSC-T6细胞增殖、Col-I有抑制作用，促进细胞外基质（ECM）降解，这可能是其抗肝纤维化的部分作用机制。

Objective To investigate the cytotoxicity of serum containing mogroside (SCM) to hepatic stellate cell (HSC)-T6 of rats and its effects on the proliferation and related gene expression. Methods HSC-T6 was cultured in vitro and treated with SCM. Lactate dehydrogenase (LDH) assay was used to evaluate the cytotoxicity of SCM; MTT assay was used to evaluate the inhibitory effect of SCM on HSC-T6; The mRNA expression of collagen alpha1 type I (Col-I), transforming growth factor-beta 1 (TGF-β1), and tissue inhibitor of metalloproteinase 1 (TIMP-1) was determined by RT-PCR. Results SCM exhibited no cytotoxicity to HSC-T6 according to the result of LDH assay, but inhibited the cell proliferation of HSC-T6 at 20% and 10% (P < 0.05, 0.01) concentration in the MTT assay significantly. From the results of RT-PCR, the mRNA expression of Col-I, TGF-β1, and TIMP-1 was significantly reduced by 20% and 10% SCMs (P < 0.05, 0.01). Conclusion SCM has no cytotoxicity on HSC-T6. SCM could inhibit the cell proliferation of HSC-T6 and Col-I, promote the degradation of extracellular matrix (ECM), which may be some parts of the mechanism of anti-hepatofibrosis.

国家自然科学基金资助项目（81160518）；广西自然科学基金创新研究团队项目（2011GXNSFF018006）；广西中医药大学中药药效研究重点实验室开放课题（09-007-06-02）