目的 通过对不同浓度NaCl胁迫下膜荚黄芪种子萌发及幼苗生理特性的研究,寻找提高膜荚黄芪种子及幼苗在NaCl胁迫下抗性能力的途径。方法 测定不同浓度NaCl胁迫下膜荚黄芪种子的发芽势(Gv)、发芽率(Gr)、相对发芽率、相对NaCl害率,并对幼苗的叶绿素量、可溶性蛋白质量、丙二醛(MDA)量、超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性进行测定。结果 低浓度的NaCl胁迫对膜荚黄芪种子的萌发具有促进作用,当NaCl胁迫浓度>50 mmol/L时,随着NaCl浓度的增大,种子的Gv、Gr、相对发芽率逐渐降低,相对NaCl害率逐渐增大,对种子萌发有抑制作用。随着NaCl处理浓度的增大,膜荚黄芪幼苗中叶绿素量逐渐减少,可溶性蛋白质量也逐渐降低,二者都与NaCl处理浓度呈负相关;随着NaCl处理浓度的增加,幼苗中MDA量呈增加趋势,呈正相关;SOD、POD活性均不同程度地表现为先上升后下降的趋势,在75 mmol/L时达到最大值。结论 膜荚黄芪具有一定的抗NaCl胁迫能力,具有向盐碱地引种驯化的潜力。

Objective To investigate the genetic diversity for germplasm resources of Premna fulva in Zhuang medicine.Methods The genetic diversity of the nine populations of P.fulva was analyzed by ISSR molecular markers.Results Thirteen primers produced 73 sites,among which 65 were polymorphic sites and Nei's genetic diversity index (H) and Shannon's information index (I) were 0.2265 and 0.3572,respectively,while average H and I at population level were 0.1476 and 0.2219.Genetic differentiation coefficient (Gst) and gene flow (Nm) were 0.348 4 and 0.935 1 within the population levels.Cluster analysis indicated that the nine populations could be divided into two groups.Conclusion The genetic diversity among the populations of P.fulva is at lower-middle level.The genetic variance of P.fulva mainly exists among the populations.The high genetic variance among the populations and the low genetic diversity within populations could be attributed to the biological characteristics of P.fulva and its region segment.

国家科技支撑计划项目“当归、黄芪、大黄种质资源可持续利用关键技术研究”(2007BAI37B01);国家中医药管理局2012年中医药行业科研专项(201207002)