目的 研究苦瓜Momordica charantia叶的化学成分。方法 采用硅胶、Sephadex LH-20等多种柱色谱方法进行分离纯化，通过理化常数和波谱分析鉴定化合物的结构。结果 从苦瓜叶95%乙醇提取物中分离得到11个化合物，分别鉴定为 (19S, 23E)-5β, 19-epoxy-19-methoxycucurbita-6, 23-diene-3β, 25-diol（1）、(19R, 23E)-5β, 19-epoxy-19-methoxycucurbita-6, 23-diene-3β, 25-diol（2）、3β, 7β, 25-trihydroxycucurbita-5, 23-dien-19-al（3）、3β, 7β, 25-trihydroxycucurbita-5, 23-dien-19-al-3- O-β-D-glucopyranoside（4）、苦瓜素I（5）、苦瓜素IV（6）、大豆脑苷I（7）、α-菠甾醇（8）、α-香树素乙酸酯（9）、β-谷甾醇（10）和胡萝卜苷（11）。结论 化合物1、3、8～11为首次从该植物中分离得到。

Objective To study the chemical constituents from the leaves of Momordica charantia. Methods The compounds were isolated and purified by column chromatography on silica gel, ODS, Sephadex LH-20, and preparative HPLC. Their structures were identified by physicochemical properties and spectral data. Results Eleven compounds were isolated from 95% ethanol extract of M. charantia and elucidated as (19S, 23E)-5β, 19-epoxy-19-methoxycucurbita-6, 23-diene-3β, 25-diol (1), (19R, 23E)-5β, 19-epoxy- 19-methoxycucurbita-6, 23-diene-3β, 25-diol (2), 3β, 7β, 25-trihydroxycucurbita-5, 23-dien-19-al (3), 3β, 7β, 25-trihydroxy- cucurbita-5, 23-dien-19-al-3-O-β-D-glucopyranoside (4), momordicin I (5), momordicin IV (6), soya-cerebroside I (7), α- spinasterol (8), α-amyrin acetate (9), β-sitosterol (10), and daucosterol (11), respectively. Conclusion Compounds 1, 3, and 8—11 are isolated from M. charantia for the first time.