目的综合比较浙江不同产区白术质量,建立白术质量评价新模式。方法采集浙江省内7个产区白术样品,采用UV法和HPLC法定量测定糖类和内酯类成分;运用主成分分析法及聚类分析法对各个指标进行综合评价。结果筛选出分别代表内酯类和糖类指标的2个主成分,特征根累积贡献率达80.376%,得到代表白术质量的综合评价函数;采用Ward’s法,利用各产区样品糖类和内酯类指标的平均值对产区进行聚类分析。将7个产区划分为3组,磐安为最优产区,於潜、龙泉次之,其他产区白术质量相对较差。结论通过主成分分析和聚类分析对不同产区白术药材质量进行综合评价,建立多指标综合质量评价模式,为白术资源的开发和临床合理用药提供理论基础。

Objective To comprehensively compare the rhizomes of Atractylodes macrocephala from different producing areas in Zhejiang Province and establish a new evaluation model. Methods A. macrocephala was collected from seven producing areas in Zhejiang Province. The contents of sugar and lactone were determined by UV and HPLC and each index was comprehensively evaluated by principal component and cluster analysis. Results Two principal components on behalf of sugar and lactone were obtained, of which the accumulated variance contribution rate was 80.376%. One comprehensive evaluation function standing for the quality of A. macrocephala was obtained. Based on the mean values of sugar and lactone indexes, seven habitats were clustered into three groups by Ward's method. Pan'an was the best among the seven producing areas, and Yuqian and Longquan were better than other producing areas. Conclusion The comprehensive evaluation on the rhizomes of A. macrocephala from different producing areas is completed using principal component and cluster analysis. The multi-index comprehensive quality evaluation model is established, which is helpful for the development of resources and rational use of A. macrocephala.

浙江农林大学研究生科研创新项目(2112010006)