目的 以铁皮石斛Dendrobium officinale带芽茎段为材料，对铁皮石斛的组织培养进行系统研究，以期建立铁皮石斛的快繁技术体系。方法 采用植物组织培养的方法对铁皮石斛外植体的消毒方法、原球茎的诱导、增殖、分化、幼苗生根及瓶苗移栽等进行研究。结果 铁皮石斛外植体最佳的消毒方式为75%乙醇消毒30 s，再用0.1% HgCl₂消毒10 min；铁皮石斛带芽茎段原球茎诱导的最佳培养基为MS＋1.0 mg/L 6-BA＋0.5 mg/L NAA＋2 g/L AC，诱导率达到34.98%；原球茎增殖的最佳培养基为MS＋0.5 mg/L 6-BA＋0.8 mg/L 2, 4-D＋2 g/L AC，增殖率达到89.6%；原球茎分化的最佳培养基为MS＋1.2 mg/L 6-BA＋0.2 mg/L IBA＋2 g/L AC，分化率达到89.6%；幼苗生根的最佳培养基配方为1/2 MS＋2.5 mg/L NAA＋15%土豆汁＋2 g/L AC，生根率达到90.4%～92.8%；瓶苗移栽最佳方式为大苗、树皮块苔藓草做基质、基质中添加0.03 mg/L赤霉素，并接种适量菌根真菌Epulorhiza sp.。结论 建立了铁皮石斛的快繁技术体系，为铁皮石斛的工业化生产奠定技术基础。

Objective Budding stems of Dendrobium officinale were used as the test materials to systematically investigate tissue culture and establish a rapid propagation technique system. Methods Disinfectant method of explants, induction, proliferation, differentiation, seedling rooting, and transplanting of protocorm in D. officinale were researched with tissue culture. Results The best disinfectant method of explants is to disinfect by 75% ethanol for 30 s and then by 0.1% HgCl₂ for 10 min. The best culture medium of budding stems induction was MS + 1.0 mg/L 6-BA + 0.5 mg/L NAA + 2 g/L AC with inductivity of 34.98%. The best culture medium of proliferation was MS + 0.5 mg/L 6-BA + 0.8 mg/L 2, 4-D + 2 g/L AC of which proliferation rate could be up to 89.6%. The best culture medium for protocorm differentiation was MS + 1.2 mg/L 6-BA + 0.2 mg/L IBA + 2 g/L AC with differentiation rate at 89.6%. The best culture medium for seedling rooting was 1/2 MS + 2.5 mg/L NAA + 15% potato juice + 2 g/L AC with rooting rate at 90.4%―92.8%. The best way for seedling transplanting was to adopt substratum of big seedling and bark moss as base with 0.03 mg/L gibberellin and Epulorhiza sp. Conclusion Rapid propagation technique system is established in order to lay technique foundation for industrial production of D. officinale.

河北省科技攻关计划项目（052201124）