目的观察当归总苯酞在活血化瘀方面的功效。方法大鼠ig给予当归总苯酞(生药1、2、4 g/kg),观察当归总苯酞对大鼠实验性动脉血栓形成及血小板聚集功能的影响;采用高分子右旋糖酐制备高黏血症大鼠模型,观察当归总苯酞对大鼠血液黏度的影响;观察当归总苯酞对大鼠凝血相关指标的影响。结果当归总苯酞(生药1、2、4 g/kg)能延长大鼠实验性动脉血栓的形成时间(P >0.05、0.01);降低高分子右旋糖苷所致的高黏血症大鼠全血、血浆、血清黏度(P >0.05);抑制花生四烯酸(AA)、腺苷二磷酸钠(ADP)、胶原(CG)诱导的血小板聚集功能(P >0.05、0.01、0.001);明显延长大鼠凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)(P >0.05、0.01、0.001)。结论当归总苯酞具有显著的活血化瘀作用,表现为延长实验性动脉血栓的形成时间;改善高黏血症模型大鼠的全血及血浆黏度;抑制血小板聚集功能;影响凝血系统,延长大鼠TT、PT、APTT等指标。

ObjectiveTo observe the effects of Angelica sinensis total phthalide (ASTP) on promoting blood circulation and removing blood stasis.Methods Rats were ig administrated with ASTP crude drug at doses of 1, 2, and 4 g/(kg·d), and the effects of ASTP on rats with experimental arterial thrombosis and platelet aggregation function were studied. Using the hyperviscosity rat model induced by polymer dextran preparation, the effects of ASTP on rat blood viscosity and coagulation related indexes were observed. ResultsASTP crude drug 1, 2, and 4 g/(kg·d) could prolong the experimental arterial thrombosis time (P< 0.05, 0.01), reduce large molecular weight dextran-induced hyperviscosity of the whole blood, plasma, and serum viscosity in rats (P< 0.05), inhibit the effects of arachidonic acid (AA), adenosine diphosphate (ADP), and collagen (CG) on platelet aggregation (P< 0.05, 0.01, and 0.001), and prolong the thrombin time (TT), prothrombin time (PT), and activate partial thromboplastin time (APTT) (P< 0.05, 0.01, and 0.001). Conclusion ASTP has remarkable effect of promoting blood circulation and removing blood stasis, which is demonstrated as extension of experimental arterial thrombosis time, improvment the whole blood and plasma viscosity of hyperviscosity rat model, inhibition of platelet aggregation function, effect on the coagulation system, and prolongation of TT, PT, and APTT indexes in rats.

国家科技支撑计划项目(2007BAI37B06)