目的 寻找发现红树林底泥放线菌(N2010-37)中抗肿瘤活性成分。方法 放线菌(N2010-37)发酵菌丝体经95%乙醇提取,溶剂分级萃取,应用多种柱色谱分离和谱学分析方法对醋酸乙酯萃取部位化学成分进行分离鉴定。结果 从醋酸乙酯部位分离得到1个新的单环内酯和2个蒽酮类化合物,依次鉴定为(3S,4R,7R,8R,9S)-3,8-二羟基-4,7,9-三甲基-2,6-环壬二酯(1)、1-甲氧基-2-羟基-3-甲基蒽醌(2)、1,6,8-三羟基-3-甲基蒽醌(3)。结论 化合物1为新化合物,体外抗肿瘤实验表明,化合物1和3对人类慢性髓性白血病细胞系K562细胞株具有一定的细胞毒活性。

Objective To study the chemical constituents in the cultured filaments of an antitumor actinomycete strain (N2010-37).Methods Compounds were isolated and purified by chromatographic techniques and recrystallization, and the structures were identified by spectral methods together with physicochemical analysis. The antitumor effects of these compounds were tested in vitro by MTT method. Results Three compounds were identified including two anthrones and one novel macrolide. They were (3S, 4R, 7R, 8R, 9S)-3, 8-dihydroxy-4, 7, 9-trimethyl-2, 6-cyclononanediiolacetone (1), 2-hydroxy-1-methoxy-3-methylanthraquinone (2), and 1, 6, 8-thihydroxy-3-methylanthraquinone (3). Conclusion Compound 1 is a new compound, and compounds 1 and 3 show the favorable cytotoxic activity against human chronic granulocytic leukemia cell line K562 strain by MTT method in vitro.

广东高校优秀青年创新人才培养计划项目资助(LYM09100);湛江师范学院自然科学研究项目博士专项(ZL1009)