目的 研究6种地被竹叶的有效成分量及其抗氧化活性。方法 分别用Folin试剂还原比色法、硝酸铝-亚硝酸钠比色法和香草醛-冰醋酸比色法测定6种地被竹叶子中总酚、总黄酮和三萜的量，采用超高效液相色谱（UPLC）定量分析荭草苷、异荭草苷、牡荆苷、异牡荆苷、对香豆酸、绿原酸、咖啡酸、阿魏酸8个特征性成分的量。同时，采用DPPH法和FRAP法调查不同竹叶提取物的抗氧化性和还原能力。结果 6种竹叶均含有较高的次生代谢产物，以干基计，总酚的质量分数为4.15%～9.12%，总黄酮为1.62%～4.00%，总萜为0.44%～0.57%；8个特征性成分在各自总酚中所占的比例依次为隈笹6.81%＞铺地竹4.80%＞翠竹4.35%，黄条金刚竹4.35%＞倭竹1.54%＞阔叶箬竹1.08%。不同地被竹种的抗氧化能力存在显著差异（P＜0.05）。结论 倭竹和黄条金刚竹的总酚和总萜量以及清除DPPH自由基的能力和还原铁离子的能力均显著高于其他地被竹。

Objective To study the effective components of the leaves in six kinds of ground bamboo (Shibataea chinensis, Pleioblastus kongosanensis f. aureostriatus, Indocalamus latifolius, Sasa pygmaea, S. argenteastriatu, and S. veitchii) and their anti-oxidant activities. Methods The contents of total phenolics, total flavonoids, and triterpenoids in bamboo leaves and their anti-oxidant activities were detected and investigated by using Forint reduction assay reagent, aluminum nitrate-sodium nitrite colorimetry, and vanillin-acetic acid assay, respectively. Eight characteristic compounds (orientin, isoorientin, vitexin, homovitexin, p-coumaric acid, chlorogenic acid, caffeic acid, and ferulic acid) were quantitatively analyzed by UPLC synchronously. The anti-oxidative and reducing activities of extracts from bamboo leaves were investigated by DPPH and FRAP. Results Six kinds of ground bamboo leaves contained abundant botanic secondary metabolites. The content of total phenolics, on dry basis of leaves, varied in a range of 4.15%―9.12% in the six kinds of ground bamboo, total flavonoids and triterpenoids located in 1.62%―4.00% and 0.44%―0.57%, respectively. The sequence of the eight characteristic compounds in total phenolics was S. veitchii 6.81% > S. argenteastriatus 4.80% > S. pygmaea 4.35%, P. kongosanensis 4.35% > S. chinensis 1.54% > I. latifolius 1.08%. The content of eight characteristic compounds and their anti-oxidant activities in each ground bamboo leaves were significantly changed (P < 0.05). Conclusion Among the six kinds of ground bamboo, there are much more effective components and more outstanding anti-oxidant activities of radical scavenging and iron reduction in two species (S. chinensis and P. kongosanensis) than those in any other banboos.

国家“十一五”支撑计划项目（2009BADB9B07）