目的观察虎杖苷对盲肠结扎穿孔(cecal ligation and puncture,CLP)所致脓毒症诱导的急性肾损伤小鼠的影响,并初步探讨其作用机制。方法建立小鼠CLP诱导的脓毒症致急性肾损伤模型,随后用不同剂量的虎杖苷(50、100、300 mg/kg)干预,观察小鼠一般情况,并于手术24 h后,用试剂盒方法测定血清尿素氮(BUN)、肌酐(Cr)水平,ELISA法测定血清白细胞介素-6(IL-6)表达量,Western blotting法检测肾脏诱导型一氧化氮合酶(iNOS)蛋白水平,并取肾脏组织进行病理分析。结果与模型组相比,虎杖苷明显改善小鼠一般情况,同时降低血清BUN、Cr、IL-6及肾脏中iNOS蛋白水平,缓解肾脏组织损伤,并呈一定的剂量依赖关系。结论虎杖苷对脓毒症致急性肾损伤具有保护作用,其作用机制可能与其抑制血清IL-6及肾脏中iNOS蛋白表达有关。

Objective To investigate the protective effect and mechanism of polydatin (PD) on sepsis-induced acute kidney injury in mice suffered from cecal ligation and puncture (CLP). Methods The mice were divided into five groups: model group, Sham group, and PD (50, 100, and 300 mg/kg) groups. The model of septic kidney injury in Kunming mice was made by CLP. One hour before the operation, PD and saline were ip administered into mice, respectively. Twenty-four hours after the operation, mice were decapitated for the measurement of urea nitrogen (BUN), serum creatinine (Cr) levels in serum using kit, and interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS) were detected by ELISA and Western blotting, respectively. Besides, the kidney tissue was assessed pathologically and kidney injury was observed histologically. Results Compared with CLP group, the levels of BUN, Cr, IL-6, and iNOS were much lowered in PD groups. In addition, PD significantly attenuated the pathologic changes in kidney tissues induced by sepsis in a dose-dependent manner. Conclusion PD has a protective effect against sepsis-induced acute kidney injury. The mechanism may be related to the inhibition on the expression of IL-6 in serum and iNOS protein in kidney tissues.

国家自然科学基金资助项目(81072650)