目的 研究大孔吸附树脂分离纯化单叶铁线莲总皂苷的工艺条件，为单叶铁线莲总皂苷的工业化生产提供实验依据。方法 采用比色法测定总皂苷；比较了5种大孔吸附树脂的静态饱和吸附量、静态解吸率，筛选出适宜的树脂；对最佳树脂进行动态吸附、解吸试验，确定最佳的吸附洗脱工艺。结果 D-101大孔树脂对单叶铁线莲总皂苷有较好的吸附分离性能。最佳工艺条件为：上样液质量浓度为生药0.2 g/mL，pH值为5，上样体积流量为1 BV/h；4 BV蒸馏水洗脱杂质；4 BV 70%乙醇洗脱总皂苷，洗脱体积流量2 BV/h。按此工艺条件生产的总皂苷质量分数达到71.53%，收率86.02%，精制度达238.81%。结论 D-101大孔吸附树脂适合富集纯化单叶铁线莲中总皂苷。

Objective To study the technological parameters of enriching and purifying total saponins from Clematis henryi by macroporous resins. Methods The content of saponins was determined by colorimetry; The optimum macroporous resins were selected by comparing static adsorptive capabilities and desorption ratio of five kinds of resins; The dynamic adsorption and desorption of D-101 resin were researched to obtain the optimum parameters. Results The D-101 resin showed the best adsorptive and desorptive capabilities; The optimum technological parameters were obtained as follows: The concentration of sample solution is crude drug 0.2 g/mL, the pH value of sample solution is 5 at flow rate of 1 BV/h; Then washed with 4 BV purified water, the desorption of saponins was washed with 70% ethanol at washing rate of 2 BV/h. The purity of total saponins obtained was 71.53%, the transfer ratio reached 86.02% and refine ratio reached 238.81%. Conclusion Using D-101 resin to enrich and purify total saponins from C. henryi is feasible.

湖南省中医药科研计划项目（2010042）