目的探讨三七止血活性与商品规格划分的相关性。方法小鼠ig给予不同商品规格三七后,测定剪尾出血时间(BT)及凝血时间(CT),并采用全自动血液分析仪测定凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(FIB)和计数血小板(PLT),同时采用HPLC法测定不同商品规格三七中三七素的量,分析三七止血活性与商品规格划分的相关性及与三七素量的关系。结果不同商品规格三七能明显缩短小鼠BT和CT、增加PLT数量、缩短PT和APTT,止血活性与商品规格划分无相关性,其中无数头三七粉剂止血效果最明显;不同商品规格三七中三七素的量存在差异,其中无数头三七中三七素质量分数最高达0.98%,80头质量分数最低仅为0.52%,与止血活性和商品规格划分无相关性。结论三七商品规格划分与止血活性及三七素的量无相关性,但是不同商品规格三七中以价格低廉的无数头三七素的量最高,也以无数头三七止血活性最佳,价格昂贵的13头和20头三七止血活性并非最好。

Objective To explore the correlation between the hemostatic activity and commercial grades of Panax notoginseng.Methods After treatment with different commercial grades of P.notoginseng,the mice blooding time(BT) and clotting time(CT) were measured.At the same time the prothrombin time(PT),activation part thrombin time(APTT),fibrinogen(FIB),and platelet count(PLT) were also detected by fully automated blood cell analyzer.The contents of dencichine were determined by HPLC.Results Mice BT,CT,PT,and APTT were decreased and PLT was increased significantly in all the commercial grades of P.notoginseng.Among all the commercial grades,the countless Tou Sanqi Powder has the best hemostatic activity.There is no correlation between the commercial grades and homostatic activity.Different contents of the extracted dencichine were observed among different commercial grades of P.notoginseng.The highest content of dencichine was 0.98% in countless Tou Sanqi and the lowest was 0.52% in 80 Tou Sanqi.There is no statistical correlation between the dencichine and hemostatic activity or dencichine and commercial grades of P.notoginseng.Conclusion There is no statistical correlation between the commercial grades of P.notoginseng and haemostatic activity or dencichine contents.The dencichine in countless Tou Sanqi which is the cheapest of all displays the better hemostaic activity than those in 13 or 20 Tou Sanqi whose cost is almost as ten times as countless Tou Sanqi’s.

国家中医药管理局基金资助项目(06-072P57);“重大新药创制”科技重大专项资助项目(2009ZX09502-022);国家公益性行业科研专项基金资助项目(200807020)