目的建立银耳多糖质量控制方法。方法采用高效凝胶渗透色谱(HPGPC)法测定银耳多糖相对分子质量及其分布和多糖的量。色谱条件:Shodex Sugar KS-804(300 mm×8 mm,7μm)和KS-805(300 mm×8 mm,17μm)凝胶排斥色谱柱串联做为分离柱,流动相为水,体积流量0.8 mL/min,柱温40℃,进样量40μL,检测条件为示差检测器。结果银耳多糖峰出现在14.40～15.30 min,对应相对分子质量为1.26×106～1.39×106。在0.01～2.0 mg/mL呈良好的线性关系(r=0.999)。银耳多糖质量浓度范围分别为:1.1～1.5 mg/mL(以透析银耳多糖为对照)和0.8～1.1 mg/mL(以蓝色葡聚糖为对照)。多糖检测限和定量限分别为0.8μg和2μg。结论所建立的方法简单、快速、准确、重现性好、灵敏度较高,可作为银耳多糖质量控制的方法。

Objective To develop a high performance gel permeation chromatography(HPGPC) method for quality control of Tremella fuciformis polysaccharide.Methods The separations were carried out with two series-wound columns including Shodex Sugar KS-804(300 mm × 8 mm,7 μm) and KS-805(300 mm × 8 mm,17 μm),water as mobile phase at flow rate 0.8 mL/min,column temperature at 40 ℃,injection volume 40 μL,and differential index detector.Results T.fuciformis polysaccharide peak was located within 14.40—15.30 min and corresponding molecular weight was 1.26 × 106—1.39 × 106.The linearity was good during 0.01—2.0 mg/mL(r = 0.999).The polysaccharide concentration was 1.1—1.5 mg/mL and 0.8—1.1 mg/mL with precipitation of T.fuciformis polysaccharide and blue dextran as reference,respectively.LOD and LOQ were 0.8 and 2 μg,respectively.Conclusion The method is simple,quick,and accurate,and has good repeatability and high sensitivity,which is fitful for quality control.

国家自然科学基金资助项目(30870551)