目的 建立铁皮石斛人工种子制作方法，并考察相关影响因素，为铁皮石斛的繁育提供一条新途径。方法 以铁皮石斛原球茎为包埋体制作人工种子，考察胚乳、种皮组分对人工种子萌发和成苗的影响。结果 以MS＋0.5 mg/L BA＋0.5 mg/L NAA＋3 g/L AC＋30 g/L海藻酸钠＋3 g/L百菌清为基本胚乳，在2% CaCl₂中反应15 min是铁皮石斛人工种子制作的较佳条件，胚乳中分别添加15 g/L木薯淀粉和10 g/L保水剂能明显提高人工种子的萌发率和成苗率，种皮中添加10 g/L纳米SiO₂和10 g/L纳米TiO₂均能明显提高萌发率，但添加10 g/L纳米SiO₂成苗率最高。结论 建立了铁皮石斛人工种子制作方法，获得了较高的萌发率和成苗率。

Objective To provide a new manufacturing method for the artificial seeds of Dendrobium officinale. Methods The artificial seeds were manufactured by taking protocorms of D. officinale as the materials entrapped with the consideration of the effects of testa components and artificial endosperm on the germination and seedling of the artificial seeds. Results The better conditions of the artificial seeds are manufactured in the fundamental artificial endosperm made by MS + 0.5 mg/L BA + 0.5 mg/L NAA +3 g/L AC + 30 g/L sodium alginate + 3 g/L chlorothalonil mixture reacted in 2% CaCl₂ for 15 min; For the higher germination and seedling rate, 15 g/L cassava starch and 10 g/L water retaining agent can be added into the artificial endosperm components, respectively; For the higher germination rate, 10 g/L nano-SiO₂ (better planting rate) and 10 g/L Nano-TiO₂ can be added into the artificial testa, respectively. The highest seedling rate was obtained by adding 10 g/L nano-SiO₂. Conclusion A manufacturing method of the artificial seeds of D. officinale has been established for the higher germination and seedling rates.

国家自然科学基金资助项目（81001601）；广东省自然科学基金资助项目（9451040701003210）；国家科技支撑计划（2011BA101B02）