目的 对云南文山西畴县的茜草科耳草属植物纤花耳草Hedyotis tenelliflora的化学成分进行系统的分离和鉴定。方法 采用色谱分离技术进行分离纯化，通过理化性质、波谱分析鉴定结构。结果 从纤花耳草的全草中分离得到了4个环烯醚萜苷化合物，分别鉴定为纤花耳草苷C（1）、哈帕苷（2）、哈帕苷元（3）、车叶草酸（4）。结论 化合物1为新化合物，化合物2和3为首次从纤花耳草中分离得到，也是首次从该属植物中得到。

Objective To study the chemical constituents of Hedyotis tenelliflora. Methods The compounds were isolated by chromatographic separation technology. The structures were identified on the basis of physicochemical and spectral data. Results Four iridoid glycosides were isolated from the whole plant of H. tenelliflora. On the basis of the chemical and spectral methods, their structures were elucidated as teneoside C (1), harpagoside (2), harpagide (3), and asperulosidic acid (4). Conclusion Compound 1 is a new compound from H. tenelliflora, and compounds 2 and 3 are found for the first time in the plants of Hedyotis L.

云南省自然科学基金资助项目（2010CD016）