目的 观察蒿鳖养阴软坚方抗CCl₄复合因素致大鼠肝纤维化的作用。方法 Wistar大鼠随机分为对照组，模型组，蒿鳖养阴软坚方低、中、高剂量组，复方鳖甲软肝片及秋水仙碱阳性对照组。CCl₄复合因素法建立肝纤维化模型，比色法测定血清丙二醛（MDA）、总蛋白（TP）、白蛋白（ALB）的量及肝组织羟脯氨酸（Hyp）水平，明胶酶谱法检测肝组织基质金属蛋白酶2（MMP-2）及基质金属蛋白酶9（MMP-9）的活性，免疫组化法检测肝组织α-平滑肌肌动蛋白（α-SMA）的表达。结果 与模型组相比，随着剂量的增加，蒿鳖养阴软坚方组大鼠血清MDA水平逐渐下降，血清TP、ALB的量逐渐增加，肝组织Hyp的量和MMP-2活性逐渐下降，MMP-9活性变化不明显，肝组织α-SMA的表达下降。结论 蒿鳖养阴软坚方能够对抗CCl₄复合因素诱发的大鼠肝纤维化。

Objective To observe the effect of Haobie Yangyin Ruanjian Decoction (HYRD) on rats with hepatic fibrosis induced by CCl₄ composited factor. Methods Wistar rats were randomly divided into seven groups: control group, model group, HYRD groups of low, medium, and high doses, positive groups of Fufang Biejia Rungan Tablets, and colchicin. Model of hepatic fibrosis was induced by CCl₄ composited factor. Malondialdehyde (MDA), total protein (TP), albumin (ALB) in serum and hydroxyproline (Hyp) in liver were detected by chromatometry; MMP-2 and MMP-9 activities in liver were detected by gelatin zymography; α-SMA expression in liver was evaluated by immunohistochemical method. Results MDA in serum and Hyp in liver were decreased gradually in HYRD groups compared with those of model group; TP and ALB in serum were increased gradually in HYRD groups compared with those of model group; MMP-2 activity in liver was decreased in HYRD groups compared with those of model group; MMP9 activity had no significant change in each group and α-SMA expression was decreased gradually in HYRD groups compared with those of model group. Conclusion HYRD can antagonize the hepatic fibrosis induced by CCl₄ composited factor.

天津市科委应用基础研究计划重点项目（06YFJZJC 02900）