目的 优选黄芪多糖脂质体制备的的最佳工艺。方法 采用薄膜分散-微孔滤膜挤压法制备黄芪多糖脂质体，以包封率和载药量为指标，以脂药比、膜材比和超声时间为因素，采用L₉(3⁴)正交试验设计对制备条件进行优选，鱼精蛋白法测定黄芪多糖脂质体的包封率，透射电镜测定其形态和粒径。结果 薄膜分散-微孔滤膜挤压法制备黄芪多糖脂质体的最佳工艺为脂药比10∶1，膜材比8∶1，超声时间20 min。结论 采用最佳工艺制备的黄芪多糖脂质体包封率和载药量较高，形态和粒径较均匀，重现性好。

Objective To optimize the preparation conditions of astragalus polysaccharide (APS) liposome. Methods APS liposome was prepared by membrane distribution-micromembrane extruding method. L₉(3⁴) orthogonal test was adopted to optimize the preparation conditions which was designed with three factors of lecithin to drug ratio, lecithin to cholesterol ratio, and ultrasonic time, and two indexes of encapsulation efficiency and drug-loading rate. The encapsulation efficiency of APS liposome was assayed by Protamine method, the shape and particle size were observed by transmission electron microscope. Results The optimized preparation conditions were as follows: the ratio of lecithin to drug was 10∶1, the ratio of lecithin to cholesterol was 8∶1, and ultrasonic time was 20 min. Conclusion The APS liposome prepared under the optimized conditions has following advantages: high encapsulation efficiency and drug-loading rate, uniform shape and particle size, and good reproduction quality.

国家自然科学基金资助项目（30901085）；国家科技支撑计划（2008BADB4B06）