目的 用NaCl模拟盐胁迫,研究接种内生真菌对菊花抗盐特性的影响. 方法 采用盆栽试验,以与内生真菌葡萄孢(C1菌株)、球毛壳菌(C4菌株)共生培养的菊花为材料,研究不同浓度盐胁迫对不同处理组菊花生理指标的影响. 结果 各处理组菊花根叶的含水量随着盐分胁迫的加重而降低,接菌处理组的根叶含水量减失程度比对照组小.各处理组SOD酶活力、可溶性蛋白量均随NaCl浓度提高而增加,20 g/L NaCl时均达到最大值,接菌处理组SOD酶活力、可溶性蛋白量高于对照组,C4组高于C1组.POD酶活力均先升高后降低,15 g/L NaCl时各处理组POD酶活力达到最大值,C4、C1组POD酶活力分别比对照组高25.50%、1.35%.15 g/L NaCl处理时,C4组PAL活力约是对照组的7倍. 结论 内生真菌增加了菊花的抗盐能力,C4组效果好于C1组.

Objective The NaCl stress conditions were simulated to study the effect of the endophytic fungi C1, C4 on antisalty characteristic of Chrysanthemum morifolium in the adverse circumstance. Methods Endophytic Botrytis sp. (C1) and Chaetomium globosum (C4) were inoculated to the C. morifolium plantlets which were planted in the pots in order to research the effects of salt stress on physiological indicators of C. morifolium. Results With the increase of NaCl concentration, the water content of root and leaf decreased in every group. The loss of root and leaf′s water in fungi-treated group was smaller than that in the control group. SOD activities in every group increased with the increase of NaCl concentration, and achieved the peak value at 20 g/L NaCl. The SOD activity in fungi-treated group was higher than that in the control group. Soluble protein of fungi-treated group was higher than that in the control group, and C4 group surpassed C1 group. POD activity increased firstly and then decreased, and compared to the control group, the POD activities in C4 and C1 groups increased by 25.50% and 1.35%, respectively at 15 g/L NaCl. PAL activity of C4 treated group was seven folds compared to the control group at 15 g/L NaCl. Conclusion Endophytic fungi could enhance the salt-tolerant ability of C. morifolium, and the effect in C4 group was better than C1 group.

国家自然科学基金项目(30770073);江苏省滩涂生物资源与环境保护重点实验室开放项目(JLCBE07005)