目的 研究灰毡毛忍冬自然群体的遗传多样性. 方法 以15个不同来源地的野生灰毡毛忍冬及2个花蕾型栽培品种为试材,通过SRAP分析,利用Popgene及Treeconw软件分析处理数据,UPGMA方法聚类,构建亲缘关系系统图. 结果 通过筛选得到24对SRAP引物,扩增出239条条带,其中210条为多态性条带,多态性条带比率为87.8%;Nei’s多样性指数He为0.239 9,Shannon多样性指数I为0.372 4;从DNA分子水平揭示出灰毡毛忍冬存在着丰富的遗传多样性.遗传相似系数在0.442 3～0.940 2.用UPGMA法得到所有样本的聚类图,可分为两大类群. 结论 灰毡毛忍冬有着丰富的遗传多样性,SRAP技术可有效运用于灰毡毛忍冬的遗传分析.

Objective To research genetic diversity of different Lonicera macranthoides geographical populations. Methods Seventeen materials were estimated by the approach of sequence-related amplified polymorphism (SRAP). The data of amplified bands were analyzed by Popgene 1.31 and Treeconw software. The system diagram of genetic relationship was built by UPGMA. Results The 24 pairs of SRAP primers combination employed produced a total of 239 discernable and reproducible amplified fragments. Among them there were 210 polymorphic bands. The percentage of polymorphic bands within different populations was 87.8%; Genetic diversity analysis showed that Nei’s gene diversity (He) was 0.239 9 and Shannon′s genetic diversity index (I) was 0.372 4. Basis on the DNA molecular level, the results indicated that there was abundant genetic diversity among the tested materials. Genetic similarity coefficient ranges changed from 0.442 3 to 0.940 2. The dendrogram including all samples was obtained by UPGMA. In the dendrogram, there were two cluster groups. Conclusion The genetic diversity of L. macranthoides geopraphical population in China is plentiful. SRAP markers can be effectively applied to genetic analysis in L. macranthoides populations.

国家“十一五”科技支撑计划(2006BAI06A12);重庆市自然科学基金资助项目(CSTC2009BB5395)