目的观察地奥心血康(DAXXK)对大鼠心肌缺血再灌注(IR)损伤的保护作用和作用机制。方法采用大鼠心肌缺血再灌注损伤模型,观察DAXXK不同剂量组心肌缺血再灌注损伤所致ECG的ST段变化、心律失常出现和持续时间、心律失常评分、心肌梗死面积和心肌形态学指标的变化。TUNEL法检测心肌细胞凋亡,免疫组化法检测凋亡相关蛋白Fax、FasL表达情况,比色法测定心肌组织抗氧化能力,RT-PCR法检测心肌Mn-SODmRNA表达,ELISA法检测血管内皮分泌功能。结果与模型组相比,DAXXK各组,ECG中ST段抬高幅度显著降低,心律失常评分明显降低,心律失常发生的开始时间明显推后,持续时间明显缩短,心肌梗死面积明显缩小,心肌细胞肿胀和炎性细胞浸润减少,心肌细胞凋亡减少,心肌总超氧化物歧化酶(T-SOD)活力增强,Mn-SODmRNA表达增加,血管内皮功能改善。上述作用呈剂量依赖性。结论 DAXXK对大鼠心肌缺血再灌注损伤的保护作用与其抗氧化损伤、抑制心肌细胞凋亡以及改善血管内皮功能有关。

Objective To observe the cardioprotective effect of Di′ao Xinxue Kang(DAXXK) and to explore the mechanisms on myocardial ischemia-reperfusion(I/R) injury in rats.Methods The myocardial ischemia reperfusion injury model was established by the ligation of left descending coronary artery for 30 min and reperfusion for 120 min in rats.By using it,the effects of DAXXK different dosage groups on the ST segment of ECG,onset and duration of ventricular arrhythmia(VA),the score of arrhythmia,and the effects on myocardial infarction size and morphologic change after myocardial injury in rats were observed.Myocardial apoptosis was detected using TUNEL method.Expressions of apoptosis-associated protein Fas and FasL were measured using the immunohistochemical method.Total-superoxide dismutase(T-SOD) was detected by spectrophotometer.NO and ET-1 in serum were detected by ELISA.Mn-SOD mRNA was measured by RT-PCR method.Results Compared with I/R group,DAXXK with dose-dependent brought down the ST segment,decreased score of arrhythmia,diminished myocardial infarction size and decreased myocardial swelling,interstitial hemorrhage,and inflammatory cell infiltration,as well as a markedly improved cardiac function.Compared with I/R group,apoptosis was decreased,positive indexes of Fas and FasL were lessened.The myocardial antioxidative activity after myocardial I/R injury was increased and vascular endothelial function was improved by DAXXK in a dose-dependent manner.Conclusion DAXXK could protect myocardium against I/R injury.The protective mechanisms could involved in the myocardial antioxidative activity,antiapoptosis action,and improvement of vascular endothelial function.