目的采用双波长HPLC法建立泽泻指纹图谱,为科学评价和控制泽泻的质量提供依据。方法色谱柱为Kromasil C18(250 mm×4.6 mm,5μm);流动相为乙腈-水;检测波长:254 nm(0~13.00 min),210 nm(13.01~60.00 min);体积流量:1.0 mL/min。结果采用双波长对福建和四川两个产地20批泽泻进行指纹图谱研究,分别确定了18和23个共有峰,并指认其中3个色谱峰。结论建立的双波长HPLC法色谱指纹图谱可以为泽泻药材的鉴别和质量评价提供依据。

Objective The HPLC dual-wavelength chromatographic fingerprint was established for evaluating and controlling the quality of Alisma orientalis.Methods Kromasil C18(250 mm×4.6 mm,5 μm) was used with acetonitrile-water gradient elution,at flow rate of 1.0 mL/min.The detection wavelength was 254 nm(0-13.00 min) and 210 nm(13.01-60.00 min).Results Fingerprint of 20 batches of A.orientalis from Fujian and Sichuan Provinces was established, and 18 and 23 characteristic peaks were identified,respectively,of which three peaks were identified.Conclusion The HPLC dual-wavelength chromatographic fingerprint method established in this study could provide the evidence for identification and quality control of A.orientalis.

国家自然科学基金资助项目(20875064)