目的通过灰色关联度分析方法系统研究了黄芪皂苷成分与环境因子之间的关系。方法采用HPLC-ELSD测定了黄芪药材中的黄芪甲苷、黄芪皂苷Ⅰ和黄芪皂苷Ⅱ,应用灰色关联度公式进行数据分析。结果灰色关联度分析表明影响黄芪甲苷的主导环境因子是年平均气温,而影响黄芪皂苷Ⅰ和黄芪皂苷Ⅱ的主导环境因子是年平均无霜期。结论为道地药材黄芪引种提供科学依据。

Objective To analyze the relationship between saponins of Astragali Radix and environmental factors by using grey correlation analysis.Methods To establish an HPLC-ELSD method for the determination of the astragaloside Ⅳ,astragaloside Ⅰ,and astragaloside Ⅱ in Astragali Radix from various habitats,the data were analyzed by using grey correlation.Results Grey correlation analysis showed the annual average temperature was the dominant climatic factor on the content of astragaloside Ⅳ.Annual average frost-free period was the dominant climatic factor on the content of astragaloside Ⅰ and astragaloside Ⅱ.Conclusion It is to provide a scientific basis for the plant introduction of Astragali Radix.

“十一五”国家科技支撑计划项目(2006BAI08B04-09);国际科技合作计划项目(2008DFA30810);“重大新药创新”科技重大专项(2009ZX09102-140)