目的建立肿节风水提物的HPLC特征图谱并明确其特征色谱峰的化学成分, 对肿节风水提物及其市售制剂的质量进行评价。方法以Ultimate XB-C₁₈色谱柱(250 mm×4.6 mm, 5μm), 乙腈-水(各含0.1%甲酸)为流动相梯度洗脱, 体积流量为1.0 mL/min, 柱温：30℃, 检测波长为330 nm;以共有模式特征图谱为对照, 对不同厂家的肿节风水提物及其市售制剂进行相似度评价。结果建立的肿节风水提物的HPLC特征图谱具有良好的稳定性、精密度、重现性。不同厂家生产的12批肿节风水提物及20批市售制剂与共有模式对照特征图谱的相似度分别在0.883~0.987和0.797~0.982。其中8个特征色谱峰经化学指认, 分别为新绿原酸、绿原酸、隐绿原酸、-βhydroxypropiovanillone、咖啡酸、异嗪皮啶、迷迭香酸-4-O-葡萄糖苷和迷迭香酸。结论本研究建立了涵盖肿节风中抗炎活性成分与特征成分的HPLC特征图谱, 反映了肿节风水提物及相关制剂的多成分的整体面貌, 为全面、有效控制肿节风及相关制剂的质量奠定了科学基础。

Objective To develop an HPLC characteristic chromatogram of Sarcandrae Herba water extract for evaluation of its quality and the related products.Methods The method was developed on a C18 column(250 mm×4.6 mm,5 μm) by gradient elution with acetonitrile-water(both containing 0.1% formic acid) as mobile phase at a flow rate of 1.0 mL/min with the detected wavelength at 330 nm and column temperature at 30 ℃.The quality of the water extract and its related products were evaluated by comparing with the established reference characteristic chromatogram.Results The HPLC characteristic reference chromatogram was established with good stability,precision,and reproducibility.Its similarity among 12 batches of the extract and 20 batches of related preparations ranged in 0.883-0.987 and 0.797-0.982,respectively.Furthermore,eight peaks in the characteristic chromatogram were identified as neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,β-hydroxypropiovanillone,caffeic acid,isofraxidin,rosmarinic acid-4-O-glucoside,and rosmarinic acid,respectively.Conclusion With anti-inflammatory bioactive ingredients and characteristic components included,the HPLC characteristic chromatogram reveales the profile of multiple chemical substances in Sarcandrae Herba water extract and its products and this would contribute to its effective quality control.

中国药典项目资助项目(TS-61);广州市中药现代化重大专项资助项目(2004Z1-E5011)