目的 以手参茎尖为外植体,研究手参体细胞胚胎发生体系。方法 应用均匀设计法筛选其最适合的胚性愈伤组织及胚性细胞复合体诱导、体细胞胚胎发育及植株再生的培养基。结果 手参茎尖胚性愈伤组织及胚性细胞复合体诱导最适宜的培养基为:CM+6-BA 1.0 mg/L+IAA 2.0 mg/L+2,4-D 0.1 mg/L,诱导率为98%;体胚发育及植株再生最适宜的培养基为:CM+6-BA 1.00 mg/L+IAA 0.10 mg/L,体胚萌发率为100%,萌发的体胚在发育培养基上继续培养50 d后全部发育成完整植株。结论 对不同阶段培养材料的形态结构及超微结构的观察证明了手参体细胞胚胎的发育过程。

Objective Using stem tip of Gymnadenia conopsea in this experiment to investigate somatic embryogenesis and plant regeneration in vitro.Methods Uniform dedign for the most suitable media for embryogenic callus induction and embryogenic cell complex,development of somatic embryo and plant regeneration were screened.Results The results showed that Chaturvedi and Mitra(CM)+6-BA 1.0 mg/L+IAA 2.0 mg/L+2,4-D 0.1 mg/L was fit for embryogenic callus and embryogenic cell complex induction.Percentage was 98%;The medium of development of somatic embryo and plant regeneration was CM+6-BA 1.00 mg/L+IAA 0.10 mg/L.Percentage was 100% and converted into plantlets with shoots and roots after 50 d culture on the same medium.Conclusion The observation of morphostructure and ultrastructure proves the process of somatic embryogenesis of G.conopsea.

国家科技攻关计划引导项目(2005BA741C)