目的 利用AFLP技术分析香鳞毛蕨种质资源多样性。方法 对46个香鳞毛蕨个体进行AFLP分析,利用NTSYS-PC 2.10、PopGene32软件分析各居群的遗传多样性。结果 香鳞毛蕨居群多态位点百分率(PPB)为76.67%;观测等位基因数(N_a)为1.766 7,有效等位基因数(N_e)为1.504 6,Ne′is基因多样性指数(H)为0.292 1,Shannon信息指数(I)为0.431 6;遗传分化指数(G_st)为0.412 5。结论 香鳞毛蕨遗传多样性较高。群体内的基因多样性(H_s)为0.170 5,群体间的基因多样性(D_st)为0.119 8,且由于生境特殊性,对香鳞毛蕨资源应采取就地保护策略。

Objective To determine the genetic diversity of germplasm resource in Dryopteris fragrans by amplified fragment length polymorphism(AFLP) technique.Methods Forty-six samples from six populations of D.fragrans were analyzed by AFLP DNA markers,and the genetic diversity was evaluated by NTSYS-PC 2.10 and PopGen32.Results The percentage of polymorphic bands(PPB) reached to 76.67%,observed number of alleles(N_a) was 1.766 7, effective number of alleles(N_e) was 1.504 6,Nei′s gene diversity index(H) was 0.292 1,Shannon information index(I) was 0.431 6,and genetic differentiation index(G_st) was 0.412 5.Conclusion Genetic diversity is high,gene diversity in populations(H_s) is 0.170 5,and gene diversity among populations(D_st) is 0.119 8.Because of environmental specificity,its resource on the spot should be protected.