目的 探讨大孔吸附树脂富集、纯化固公果中总鞣质的工艺条件参数。方法 比较了8种大孔吸附树脂的静态吸附量、静态解吸率,筛选出最佳的吸附树脂。以静态、动态吸附实验相结合,确定了最佳的吸附分离工艺。采用干酪素法进行鞣质的测定。结果 在考察的8种树脂中,HP20型树脂具有最佳的吸附参数,并确定了工艺条件。结论 本实验筛选出HP20型树脂对固公果根中总鞣质类成分吸附解吸性能较好。

Objective To study the technological parameters in the purification process of total tannins from the root of Rosa odorata var.gigantea by macroporous resin.Methods Using adsorptive quantity as the guideline,the adsorptive capabilities and static desorptim ratio of eight kinds of resins were compared,and the optimum macroporous resins were selected.The influence factors of total tannins purification from the root of R.odorata var.gigantea with the optimun macroporous resin were tested and screened by the adsorption and desorption ratio of total tannins.The content of tannins was determined by casein method.Results The HP20 type macroporous resin had the optimum adsorption and desorption parameters,and the optimum purification conditions were determined.Conclusion The HP20 type macroporous resin shows better comprehensive property and could be used to isolate and purify the total tannins from the root of R.odorata var.gigantea.

天津市应用基础及前沿技术研究计划项目(10JCYBJC14600)；武警医学院博士启动金项目(WBS200804)