目的 研究碳源、氮源和金属离子对秦艽悬浮细胞生长及龙胆苦苷积累的影响。方法 测定不同浓度蔗糖和葡萄糖、不同NH₄⁺/NO₃^-比及Ca²⁺、Zn²⁺影响因素下细胞干质量和龙胆苦苷量。结果 有利于秦艽悬浮细胞生长及龙胆苦苷积累的培养条件为:3%蔗糖,NH₄⁺/NO₃^-为20/40,3mmol/LCa²⁺,0.00749mmol/L Zn²⁺。结论 不同碳源及其浓度对细胞生长及龙胆苦苷积累都有显著影响,高糖不利于龙胆苦苷的合成。NO₃^-对细胞生长来说,是一种比NH₄⁺更好的氮源。Ca²⁺对细胞生长和龙胆苦苷的量都有显著影响,Zn²⁺对细胞生长影响不明显,对龙胆苦苷量影响显著。

Objective To investigate the effects of〔arbohydrate and its concentration,nitrogen source,and metal ions on suspension cell growth of Gentiana macrophylla and gentiopicroside accumulation.Methods The cell dry weight and gentiopicroside content were measured under factors of different carbohydrate,NH₄⁺/NO₃^-，Ca²⁺，and Zn²⁺ at various concentration.Results The optimum culture condition are as follows:3% sucrose,NH₄⁺/NO₃^-of 20/40,3 mmol/L Ca²⁺，0.00749 mmol/L Zn²⁺.Conclusion Different carbohydrates and their concentration have significant effects on cell growth and gentiopicroside accumulation while high concentration of carbohydrates does not have such effect.NO₃^- is better than NH₄⁺ for the cell growth.Ca²⁺ Remarkably influences both cell growth and gentiopicroside accumulation while Zn²⁺ contributes only to gentiopicroside content.

西安市科技局项目(YF07118)