目的 研究三草方(Sancaofang,SCF)不同组分及其配伍体内外抗肺癌的作用,筛选出有效的抗癌组分并确定最合适的配伍方案,探索SCF抗肺癌的物质基础。方法 采用不同体积分数乙醇提取,经大孔树脂纯化得黄酮类(D)、萜类(E)及其他类组分(A+B+C);体外实验采用两种人肺癌细胞株SPC-A-1和A549,以MTT法测定不同组分及其配伍对细胞增殖活性的影响;体内实验采用接种Lewis肺癌细胞的C57BL/6J小黑鼠,作为药效的动物模型,以抑瘤率、脾指数、胸腺指数为指标进行药效评价。结果 SCF黄酮类、萜类组分对两种细胞有较强的抑制作用,组分E_Ⅰ、D_Ⅱ及E_Ⅱ对SPC-A-1细胞的IC₅₀分别为生药量6.35、7.71、6.75mg/mL,对A549细胞的IC₅₀分别为生药量8.05、6.79、6.88mg/mL;黄酮类和萜类组分配伍(D_Ⅰ+D_Ⅱ+E_Ⅰ+E_Ⅱ)对Lewis小鼠肿瘤生长具有抑制作用,且可提高荷瘤小鼠的胸腺指数。体内外实验结果表明,黄酮类、萜类组分配伍后具有明显的协同抑瘤活性。结论 SCF黄酮类、萜类组分间的配伍为最佳配伍,SCF有效组分具有良好的抗肺癌应用前景。

Objective To study the in vivo and In vitro anti-lung cancer activity of Sancaofang(SCF) components and their compatibility，screen the most effective components of anti-tumor，ascertain the best suitable compatibility program，and research the anti-tumor material base of SCF.Methods Alcohol parts (95%and 60%)were prepared and SCF components were separated by macroporous resin;SPC-A-1 and A549 cell models were adoptedfor an vitro experiment and MTT assay was used;C57 BL/6J black mice injected with Lewis cells were adopted for the an vivo experiment;and tumor inhibition ratio，spleen index，and thymus index were used for the evaluation.Results Components E_Ⅰ，D_Ⅱ，and E_Ⅱ had comparative strong anti-tumor activity against SPC-A-1 and A549 cells and IC₅₀ was relatively low;The in vivo and In vitro experiments showed that component compatibility of D and E had the apparent synergism anti-tumor activity.Conclusion The most suitable compatibility program is the compatibility of component D and E,the components of SCF will have prospect application to anti-tumor.

江苏省中医药领军人才项目(2006);江苏省自然科学基金资助项目(BK2006155)