目的建立自主活动实时测试分析处理系统,并用此系统检测安神中药复方开心散对小鼠自主活动的影响。方法将计算机、信息和图像分析技术集成用于动物自主活动测试实验中,在线分析获取大鼠、小鼠自主活动的信息资源,采用模式识别、数据挖掘等技术对信息资源进行科学组合和深层次开发,提取包括运动距离、运动时间、运动速度、静息时间及各种比值以及运动轨迹等快速灵敏、规范客观的评价指标;利用正常小鼠、安定和咖啡因造模的小鼠、开心散中药复方,对系统的稳定可靠性进行实验研究。结果该系统用于评价动物自主活动的指标客观灵敏,所获数据与原人工检测方法所获结果具有高度相关性,r>0.9;该系统有很好的重现性和稳定性。结论自主活动实时测试分析处理系统集各种信号监测、采集、自动判别、贮存、结果分析、统计处理和输出一体化,为安神药物的实验研究提供了一套自动化程度高、提取信息量大、可供推广应用的自主活动实时测试分析处理系统。

ObjectiveTo set up a system for computer-based image-processing for animal's locomotor activity test in sedative Chinese materia medica(CMM)，Kaixin Powder, one of traditional complex prescription. MethodsCombining the advantage of computer sciences，information knowledge，and imaging analyzed technique into the open field test,the data of mice locomotor activities were collected and analyzed with technique of pattern-recognition,data mining，etc.The total distance,time and speed of movement，resting time and their ratios in the open field were developed;and those various indexes in central area and peripheral area in the open field were established.The system was also verified by using Kaixin Powder.Results Those indexes were sensitive and objective for judging animal's locomotor activity.The correlation coefficient of those indexes collected by computer and manpower was over 0.9，which indicated that the system had better reproducibility and stability.Conclusion The results show that the system could provide an automatic and intelligent method for studying sedative CMM with integration for monitor,collection,analysis，storage,statistics，and output of experimental data and results.

国家人事部中医药回国人员重点基金资助项目(2003LHR01);国家科技部国际科技合作重点资助项目(2006DFA21740)