[关键词]
[摘要]
目的探讨七叶皂苷钠对小鼠急性淋巴白血病L1210细胞的体外增殖抑制作用和体内抗白血病作用。方法采用MTT法观察七叶皂苷钠对小鼠急性淋巴白血病细胞L1210的体外增殖抑制作用;建立L1210小鼠白血病移植模型,观察七叶皂苷钠对荷瘤小鼠生命的延长率,以及对化疗药阿霉素的增敏作用,并绘制生存曲线。结果七叶皂苷钠(20~60μg/mL)对L1210细胞具有一定的增殖抑制作用,作用呈时间和剂量依赖性,体外作用72 h的IC50为(24.86±2.23)μg/mL;体内实验结果显示,七叶皂苷钠中、高剂量组能明显延长荷瘤小鼠的生命期,生命延长率分别为32.3%和25.8%(P<0.01),低剂量组对常规化疗药阿霉素具有增敏作用。结论七叶皂苷钠能有效抑制L1210细胞体外增殖,对体内L1210白血病小鼠移植模型也具有一定的治疗效果。
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[Abstract]
ObjectiveTo explore aescin' s inhibition of in vitro proliferatim in L1210 murine acute lymphoid leukemia cells and its in vivo antileukemic effects.Methods MTT assay was used to determine the anti-proliferative effect in vitro;Leukemia transplant models were used to test the antileukemic effect.Mice inoculated with L1210 cells were treated with ten daily doses of aescin or/and each other day of DOX,and were observed for survival.Antileukemic effects were assessed by calculating the extension of lifespan.Results Aescin (20一60 p.g/mL) was found to be able to inhibit the proliferation of L1210 cells. The effects were in a dose-and time-dependent manner.The IC50, value of aescin in L1210 cells after 72h was (24.86±2.23) ug/mL.In vivo study showed that after treating the L1210 cells bearing mice with higher,middle,and low dosage(4:5,3.5,and 2.5 mg/kg) of aescin for ten consecutive days,The extension of lifespan were 25. 8%(P<0.O1),32.3%(P<0.0l),and 12.9%,respectively.Moreover, the extension of lifespan was 45.2%when mice were treated with a low dose of aescin in combination with DOX(2mg/kg),which was significantly increased compared with 29.0% when treated with singleDOX (P<0.01). Conclusion The data presented here indicate that aescm is a poent natural inhibitor of proliferation in L1210 lymphoid leukemia cells and aesctn has the potential to be a promising candidate for cancer treatment.
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[基金项目]
浙江省自然科学基金项目(Y205636);浙江省科技厅项目(2008C23064)