目的建立测定辛夷挥发油软胶囊中α-蒎烯、β-蒎烯、柠檬烯和1,8-桉叶素的测定方法。方法采用毛细管气相色谱法测定。BP-5毛细管柱(30 m×0.25 mm,0.22μm);进样器温度180℃;FID检测器温度200℃;柱温采用程序升温;分流比1∶30;苯乙酮为内标。结果α-蒎烯在0.054~2.505μg线性关系良好,平均加样回收率为98.6%,RSD为0.72%(n=9);β-蒎烯在0.070~3.204μg线性关系良好,平均加样回收率为99.0%,RSD为0.65%(n=9);柠檬烯在0.068~3.190μg线性关系良好,平均加样回收率为101.1%,RSD为0.56%(n=9);1,8-桉叶素在0.301~10.205μg线性关系良好,平均加样回收率为99.6%,RSD为1.24%(n=9)。结论该方法简便、准确、重现性好,可作为辛夷挥发油软胶囊的质量控制方法。

Objective To develop a method for determination of α-pinene,β-pinene,limonen,and 1,8-cineole in Flos Magnoliae volatile oil soft capsula.Methods The contents of α-pinene,β-pinene,limonen,and 1,8-cineole were determined at the same time by capillary gas chromatography with sequential increase of temperature programming.Capillary column BP-5(30 m × 0.25 mm,0.22 μm) was used,injector temperature 180 ℃,flame ionization detector(FID) temperature 200 ℃,split ratio 1∶30,acetophenone as internal standard.Results A good linear relationship of α-pinene was at a range of 0.054—2.505 μg,the average recovery was 98.6 %,and RSD was 0.72 %(n=9);A good linear relationship of β-pinene was at a range of 0.070—3.204 μg,the average recovery was 99.0 %,and RSD was 0.65%(n=9);A good linear relationship of limonen was at a range of 0.068—3.190 μg,the average recovery was 101.1 %,and RSD was 0.56%(n=9);A good linear relationship of 1,8-cineole was at a range of 0.301—10.205 μg,the average recovery was 99.6%,and RSD was 1.24%(n=9).Conclusion The method is simple and accurate one with good reproducibility and could be used for the quality control of Flos Magnoliae volatile oil soft capsula.

国家“973”项目(2006CB504707)