目的研究大孔树脂纯化山蜡梅叶中总黄酮的工艺条件及参数。方法采用静态吸附-解吸方法,以吸附量和解吸率为指标,筛选最佳树脂;又以总黄酮质量浓度为指标,考察了最佳树脂纯化山蜡梅叶中总黄酮的工艺参数。结果8种树脂中,HPD400树脂对山蜡梅叶中总黄酮纯化效果较好,其总黄酮的静态吸附量达到17.77 mg/g树脂,解吸率为92.24%;动态吸附量为1.68 g/g树脂,用4倍柱体积蒸馏水、4倍柱体积30%乙醇洗脱除去杂质后,换用70%乙醇6倍柱体积洗脱,总黄酮质量分数为31.4%,总黄酮转移率为88.4%。结论HPD400型大孔树脂在所确定的工艺条件下,可较好地纯化山蜡梅叶总黄酮。

Objective To investigate the technological parameters of the purification process of total flavones from the leaves of Chimonanthus nitens with macroporous adsorption resins.Methods Taking the static elution ratio and static absorption ratio as indexes,the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index.Results The HPD400 macroporous resin among eight types of resins was found with the best separating efficiency,by which the dynamic absorption capacity could reach 1.68 g(crude drug)/g.Static absorption capacity and desorption ratio were 17.77 mg/g and 92.24%,respectively.After eluted with 4 BV of distilled water and 4 BV of 30% ethanol,eluant was changed with 6 BV of 70% ethanol,the yield of total flavones was 31.4% and product purity was 88.4%.Conclusion With definite technical process,the HPD400 macroporous adsorption resin could be used to purify the extract in the leaves of C.nitens.

国家“十一五”科技支撑计划课题(2006BAI06A18-09,2006BAI06A01-01);江西省卫生厅中医药科研计划课题(2008A060)