目的研究羽芒菊Tridax procumbens的化学成分。方法利用Diaion HP-20、MCI-Gel CHP-20、Sepha-dex LH-20及硅胶等柱色谱法对羽芒菊中的化学成分进行分离纯化,根据理化性质和波谱技术(¹H-NMR、¹³C-NMR、DEPT、¹H-¹H COSY、HSQC、HMBC、ESI-MS、IR)鉴定化合物的结构。结果从中分离鉴定了5个化合物:8,3′-二羟基-3,7,4′-三甲氧基-6-O-β-D-葡萄糖黄酮苷-6″-乙酸酯(Ⅰ)、豆甾醇(Ⅱ)、β-谷甾醇(Ⅲ)、邻苯二甲酸二-(2-乙基)己酯(Ⅳ)、芹菜素-7-O-β-D-葡萄糖苷(Ⅴ)。结论化合物Ⅰ为新化合物,命名为羽芒菊苷A(trid-procumoside A),化合物Ⅱ~Ⅴ为首次从该植物中分离得到。

Objective To study the chemical constituents in Tridax procumbens from Hainan Province.Methods The constituents were separated and purified by column chromatography with macroporous adsorption resin Diaion HP-20,MCI Gel CHP-20,Sephadex LH-20,and normal phase silica gel.Their structures were identified on the basis of physiochemical properties and spectral methods(¹H-NMR,¹³C-NMR,DEPT,¹H-¹H COSY,HSQC,HMBC,ESI-MS,and IR).Results Five compounds were isolated and identified as:8,3'-dihydroxy-3,7,4'-trimethoxy-6-O-β-D-flavone glycoside-6″-acetate(Ⅰ),stigmasterol(Ⅱ),β-sitosterol(Ⅲ),bis(2-ethylhexyl)phthalate(Ⅳ),apigenin-7-O-β-D-glucoside(Ⅴ).Conclusion Compound Ⅰ is a new compound,named tridprocumoside A,the other compounds are isolated from this plant for the first time.

浙江省森林培育重中之重学科开放基金项目(200607)