目的 分离和鉴定见血封喉Antiaris toxicaria 根韧皮部中的内生真菌,并检测其抑菌活性。方法 以金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林金黄色葡萄球菌methicillin-resistant Staphylococcus aureus(MRSA)和白色念珠菌Candida albicans 作为指示菌,采用杯碟法对内生真菌发酵上清液进行抑菌试验。结果 从见血封喉根的韧皮部中分离出6株内生真菌,经形态鉴定分属于聚端孢霉属(Trichothecium LK.ex Fr.)、枝顶孢霉属(Acremonium LK.ex Fr.)和丝核菌属(Rhizoctonia DC.ex Fr.)。其中内生真菌J 6的发酵上清液对MR-SA 有抑菌活性,抑菌圈直径为18mm。结论 首次对见血封喉内生真菌进行了研究,内生真菌J 6对MRSA 有明显的抑菌活性,其抑菌活性成分值得进一步研究。

Objective To isolate and identify endophytic fungi from the phloem in the root of Rhizoctonia and detect the antimicrobial activity of these strains.Methods Staphylococcus aureus,methicillin-resistant Staphylococcus aureus(MRSA),and Candida albicans were selected as indicators.The antimicrobial activities of the fermentation filtrate of endophytic fungi were tested by cylinder plate method.Results Six endophytic fungi were isolated from the phloem in the root of A. toxicaria.These strains were morphologically identified to genus of Trichothecium LK.ex Fr.,Acremonium LK.ex Fr.and Rhizoctonia DC.ex Fr.The fermentation filtrate of endophytic fungus J6 showed the antimicrobial activity against MRSA.The diameter of inhibition zone was 18mm.Conclusion It′s the first report about the endophytic fungi from A. toxicaria.Endophytic fungus J6 exhibits strong antimicrobial activity against MRSA,its active components are of worth to further study.

973计划前期研究专项(2007CB116306);海南省自然科学基金资助项目(30608);海南省自然科学基金指导性项目(80671)