目的 进行药用植物桃儿七Sinopodophyllum hexandrum 的遗传多样性研究,为桃儿七资源的保护和开发利用提供分子遗传理论基础。方法 对6个桃儿七居群进行DALP 分析,利用PopGene 1.31软件分析遗传多样性相关数据,UPGMA 方法聚类,结合Treeview 软件生成树状图。结果 筛选5对引物组合,扩增共产生150条DNA 片段,其中104条谱带具有遗传多态性,占69.33%,平均每组引物扩增所得多态条带为20.8,6个居群平均多态百分率为14.22%。6居群总的观察等位基因数Na 为1.6933,平均1.1422;总的有效等位基因数Ne为1.4171,平均1.0838;基因多样性指数H 为0.2443,平均0.0488;总的Shannon 多样性指数I 为0.3643,平均0.0730;居群总的基因多样性Ht 为0.2443,居群内基因多样性Hs 为0.0487。基因分化系数Gst 为0.8005,即遗传变异有80.05%发生在居群间,19.95%发生在居群内,居群每代迁移数Nm 为0.1246。结论 桃儿七居群间存在较大的遗传分化,基因流受到阻碍,这与桃儿七的繁育系统和生存环境紧密相关。

Objective To study the genetic diversity of Sinopodophyllum hexandrum so as to provide basic molecular genetic evidence for protecting and exploiting the resource of S. hexandrum.Methods Six populations of S. hexandrum were analyzed by DALP molecular markers.To make up the genetic diversity correlation data by PopGene 1.31 software and cluster by UPGMA method,and establish the dendrogram.The tree was subsequently visualized with Treeview software.Results Five primer groups were screened and a total of 150 DNA fragments were amplified,among which 104 were polymorphic,i.e.the percentage of polymorphic bands(PPB) was 69.33%,the average number of DNA polymorphic band amplified by each primer group was 20.8 and the average PPB was 14.22%.In the six populations,the total observed number of alleles(Na) was 1.6933 and the average Na was 1.1422;The total effective number of alleles(Ne) was 1.4171 and the average Ne was 1.0838;The total Nei′s gene diversity(H) was 0.2443 and the average H was 0.0488;The total Shannon′s information index(I) was 0.3643 and the average I was 0.0730;The total gene diversity(Ht) was 0.2443 while the gene diversity within a population(Hs) was 0.0487.The coefficient of gene differentiation(Gst) was 0.8005 between populations,namely 80.05% genetic variation occurring between populations and the rest 19.95% within population,and estimated gene flow(Nm) was 0.1246.Conclusion The result indicats that S. hexandrum has much larger genetic differentiation among populations and gene flow has been blocked.This might be a result of species breeding system and population habitat.

国家自然科学基金资助项目(30370156);国家科技基础条件平台项目(2005DKA21000-5-09)