目的 优选丹参水提液纯化工艺。方法 以丹参提取液的浊度、鞣质以及有效成分的量为指标,优选两种絮凝澄清剂的用量和絮凝搅拌速度,筛选出较佳参数;对比研究醇水沉淀、絮凝结合乙醇沉淀、絮凝结合膜过滤3种纯化方法。结果 使用壳聚糖进行絮凝澄清处理后的丹参水提液上清液浊度低于用ZTC1+1Ⅱ 型絮凝剂处理后的浊度,并能够更好地减轻膜污染程度;壳聚糖结合膜过滤法对丹参水提液的有效成分保留率较高,收膏率相对较高,浊度和鞣质含量无显著差异。结论 壳聚糖作为丹参水提液絮凝澄剂效果优于ZTC1+1Ⅱ 型絮凝剂,壳聚糖结合膜过滤法纯化丹参水提液的综合评价指标优于其他两种方法。

Objective To optimize the purifying process of Danshen water extract solution. Methods Taking the turbidity, content of tannin, and active components in the Danshen water extract solution as index, to optimize the better flocculant and its best flocculat stiring speed. The purifying effects of alcohol-water sedimentation, flocculation binding alcohol-water sedimentation, and flocculation binding membrane filtration were studied. Results The turbidity of the chitosan-treated supernatant is lower than that of ZTC1+ 1ò-treated ones, and this treatment could mitigate the membrane fouling better. Chitosan binding membrane filtration had a better retention rate and higher yield of active components, and there was no significant difference in the turbidity and the tannin content. Conclusion Chitosan as the flocculant of the Danshen water extract solution is superior to ZTC1+ 1ò. Chitosan binding membrane filtration is more effective than alcohol sedimentation and flocculation binding alcohol-water sedimentation in retaining the active components and comprehensive evaluation index.

科技部科技型中小型企业技术创新基金(07C26215100788)