目的 建立了紫外检测的快速灵敏的高效液相色谱法,用于测定犬血浆中的丹参素钠。方法 血浆样品经液-液萃取后,以原儿茶酸为内标,采用Discovery C₁₈ 色谱柱进行分离,HPLC 测定犬血浆中的药物浓度。结果 犬分别静脉滴注给予10、20、40mg/kg 丹参素钠后,AUC_0-t、AUC_0-∞、C_max 均与给药剂量呈线性相关。AUC_0-t、AUC_0-∞ 的相关系数分别为0.964、0.964。C_max 的相关系数为0.9347;丹参素钠在犬体内的药动学过程符合二室模型。结论 通过方法学确证,该方法可以应用于丹参素钠在犬血浆中的药物动力学研究。

Objective To develop a high-performance liquid chromatographic method for the determination of sodium danshensu in dog plasma.Methods The samples were extracted from plasma by liquid-liquid extraction,separated through a Discovery C₁₈ column,and protocatechuic acid was used as the internal standard in determination of sodium danshensu in dog plasma by HPLC.Results After iv administration of sodium danshensu to dogs at doses of 10,20,and 40mg/kg,respectively,the AUC_0-t,AUC_0-∞,and C_max dose relationships were evaluated to be linear(r=0.964,0.964,and 0.9347).The results of the compartmental analysis indicated that the kinetic process of sodium danshensu in dogs in vivo was best fitted to a two-compartment model.Conclusion The method is successfully applied to defination of the plasma concentration-time curves after iv drop administration of sodium danshensu to dogs.