目的 利用高效液相色谱建立温郁金挥发油的指纹图谱。方法 采用Shimadzu VP-ODS C₁₈ 色谱柱(250mm×4.6mm,5μm),以乙腈-0.1磷酸溶液梯度洗脱,体积流量为1.0mL/min,柱温30℃,检测波长210nm。结果 以牻牛儿酮对照,通过对19批浙江不同产地温郁金样品测定,标定了14个共有峰,并通过"中药色谱指纹图谱相似度评价系统软件"和聚类分析等辅助手段,计算相似度等有关参数。结论 该方法准确可靠,重现性好,为更好的控制温郁金内在质量提供了科学依据。

Objective To establish the chromatographic fingerprint of Curcuma wenyujin by HPLC.Methods The chromatographic fingerprint of C. wenyujin was established by HPLC with Shimadzu VP-ODS C₁₈ column(250mm×4.6mm,5μm),acetonitrile-water(0.1 phosphoric acid) gradient elution,the flow rate of 1.0mL/min,column temperatures of 30℃,and detective wavelength at 210nm.Results Taking germacrone as the reference peak,14 common-peaks were selected as the fingerprint peaks.The similarities among samples of C. wenyujin among 19 approved samples and their standard chromatographic fingerprints were calculated with a software "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" published by GPC(Version 2004A) and cluster analysis.Conclusion This method with better accuracy and reproducibility could be used in the quality control of C. wenyujin.

浙江省科技攻关重大项目(2005C13019);瑞安市重大科技资助项目(20052005);温州市科技计划资助项目(S2005B001)