目的探讨地黄连作障碍的原因和筛选可能具有缓解作用的有益菌.方法将已分离纯化的土壤细菌菌株接种到试管苗和盆栽苗基部,分别于30 d和60 d后收获,测量苗质量等指标.结果组培试验表明48个菌株中,有11个对地黄生长具有显著促进作用,另外11个具有抑制或致死作用;盆栽试验揭示16个菌株对地黄苗具有促进作用,13个菌株具有抑制或致死作用.结论土壤细菌对地黄苗的生长具有显著影响.地黄种植可能导致根际细菌菌群失衡,施用有益细菌可能是解决地黄连作障碍的有效措施.

Objective To investigate the cause of continuous cropping obstacle ofRehmannia glutinosa and screen beneficial bacteria.Met-hods In vitro cultured plantlets and potted plants were inoculated with different isolated soil bacteria.The plants were harvested and weighted in 30 and 60 d,respectively.Results In thein vitroculture experiment,1.out of 48 strains displayed promoting act-ion on the growth of plantlets,and 1.other strains showed inhibitory or lethal action.In the potted test,16 strains showed promoting action and 13 strains showed suppressing or lethal action.Conclusion Soil bacteria influence the growth ofR.glutinosa significantly. The flora of rhizosphere bacteria may be disturbed by the cultivation ofR.glutinosa and inoculation of beneficial bacteria might be effective on the resolution of continuous cropping obstacle ofR.glutinosa.

国家自然科学基金资助项目(30572326);国家“十一五”支撑计划项目(2006BA109B03)